Stack G, Gorski J
Endocrinology. 1985 Nov;117(5):2017-23. doi: 10.1210/endo-117-5-2017.
Multiple injections of a short acting estrogen, 1,3,5(10)-estratriene-3,16 alpha-diol (16 alpha-E2), have been used to analyze the lag or prereplicative period of approximately 12 h, which precedes the onset of estrogen-stimulated DNA synthesis. A single injection of 1.0 microgram 16 alpha-E2, which itself does not stimulate DNA synthesis, shortened by 3-4 h the lag period between subsequently administered estrogen and the initiation of DNA synthesis. This lag-shortening effect of 16 alpha-E2 was stable for 24 h, but had decayed by 36 h. One or two additional injections of 16 alpha-E2 given sequentially at 3-h intervals each further shortened the lag period but to a lesser extent than the first injection. The results indicate that estrogen induces the accumulation of relatively stable cell changes which are required for the onset of DNA synthesis. The prolonged estrogen requirement during the lag period is not truly discontinuous as previously suggested but rather can be satisfied by discontinuous pulses of estrogen in a ratchet-like fashion because of the stability of their effects.
多次注射短效雌激素1,3,5(10)-雌三烯-3,16α-二醇（16α-E2）已被用于分析雌激素刺激的DNA合成开始前约12小时的滞后或复制前期。单次注射1.0微克16α-E2本身不会刺激DNA合成，但可将随后给予雌激素与DNA合成起始之间的滞后时间缩短3 - 4小时。16α-E2的这种滞后缩短效应在24小时内稳定，但在36小时时已衰减。每隔3小时依次再注射一到两次16α-E2可进一步缩短滞后时间，但程度小于首次注射。结果表明，雌激素诱导了DNA合成开始所需的相对稳定的细胞变化积累。滞后期间对雌激素的长期需求并非如先前所认为的那样真正不连续，而是由于其作用的稳定性，可以通过类似棘轮的方式由不连续的雌激素脉冲来满足。
