Ye Jiayu, Raman Maitreyi, Taylor Lorian M, Yousuf Munazza, Panaccione Remo, Turbide Christian, Sinha Sidhartha R, Haskey Natasha
Division of Gastroenterology and Hepatology, Stanford Medicine, Stanford University, 300 Pasteur Dr., Palo Alto, CA 94305, USA.
Department of Medicine, Cumming School of Medicine, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Int J Mol Sci. 2025 May 11;26(10):4596. doi: 10.3390/ijms26104596.
This pilot study investigated the effects of a reduced sulfur (RS) diet on the gut microbiome composition and fecal metabolome in individuals with remitted or active ulcerative colitis (UC). Thirteen participants maintained their habitual diet (control), while nine followed an RS diet for eight weeks (Wk8). Stool and plasma samples were collected at the baseline and Wk8. The sulfur intake decreased in the RS group (-28 g/1000 kcal) versus the control group (-1.7 g/1000 kcal; < 0.001). The RS group exhibited a significant decrease in lipopolysaccharide-binding protein (-5280 ng/mL), while these levels increased in the control group (620 ng/mL; < 0.05). The microbiome analysis showed an increased alpha diversity at Wk8 ( < 0.01), suggesting a microbial shift with a RS intake. The metabolic alterations indicated enhanced nitrogen disposal (increased uric acid, methyluric acid, N-acetyl-L-glutamate) and a higher energy demand (elevated ubiquinol and glucose-pyruvate). The RS diet increased beneficial microbes , , and , while decreasing pathobionts and . Methyluric acid correlated positively with (β = 0.70) and negatively with (β = -0.77) suggesting these microbes utilized this metabolite and influenced the microbiome composition. In conclusion, a RS diet promoted microbial diversity, metabolic adaptations, and reduced inflammation, highlighting its potential as a novel strategy for UC management.
这项初步研究调查了低硫（RS）饮食对缓解期或活动期溃疡性结肠炎（UC）患者肠道微生物群组成和粪便代谢组的影响。13名参与者维持其习惯饮食（对照组），而9名参与者遵循RS饮食8周（第8周）。在基线和第8周收集粪便和血浆样本。与对照组（-1.7 g/1000 kcal；<0.001）相比，RS组的硫摄入量下降（-28 g/1000 kcal）。RS组的脂多糖结合蛋白显著降低（-5280 ng/mL），而对照组的这些水平升高（620 ng/mL；<0.05）。微生物群分析显示，在第8周时α多样性增加（<0.01），表明随着RS摄入量的增加微生物发生了变化。代谢改变表明氮处理增强（尿酸、甲基尿酸、N-乙酰-L-谷氨酸增加）和能量需求增加（泛醇和葡萄糖-丙酮酸升高）。RS饮食增加了有益微生物、和，同时减少了致病共生菌和。甲基尿酸与呈正相关（β=0.70），与呈负相关（β=-0.77），表明这些微生物利用了这种代谢物并影响了微生物群组成。总之，RS饮食促进了微生物多样性、代谢适应并减轻了炎症，突出了其作为UC管理新策略的潜力。
