黑腹果蝇线粒体DNA的分子内异质性
Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster.
作者信息
Goldring E S, Peacock W J
出版信息
J Cell Biol. 1977 May;73(2):279-86. doi: 10.1083/jcb.73.2.279.
Abstract
DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species.
摘要
从黑腹果蝇纯化的线粒体中分离出的DNA可以作为超螺旋分子被分离出来，这些分子在切口时的轮廓长度为5.9微米。部分变性图谱显示碱基组成存在区域异质性，有一个早期变性区域，计算得出的GC含量接近零，延伸超过基因组的20%。从未受精卵中分离出的DNA显示核DNA和线粒体DNA比例相等；我们没有发现其他细胞质物种的证据。
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