黑腹果蝇中DNA的特征分析。
Characterization of the DNA in Drosophila melanogaster.
作者信息
Travaglini E C, Petrovic J, Schultz J
出版信息
Genetics. 1972 Nov;72(3):419-30. doi: 10.1093/genetics/72.3.419.
DNA has been quantitatively extracted from Drosophila melanogaster at various stages of embryonic development and analyzed by isopycnic centrifugation in CsCl and by fractionation on methylated albumin columns. The DNA is composed of three main classes of DNA, as defined by their buoyant density, rho, in CsCl: a bulk DNA, rho = 1.699 g cm(-3), and two satellite DNAs, rho = 1.685 g cm(-3) and rho = 1.669 g cm(-3). These three types of DNA persist throughout the development of the insect. In the unfertilized egg, 80% of the total DNA consists of the satellite DNAs; this amount decreases to 18% during the first three hours after fertilization and then remains constant through embryogenesis. There is a concomitant increase of the satellite DNA's with the bulk DNA after blastoderm formation.
已从黑腹果蝇胚胎发育的各个阶段定量提取DNA，并通过在CsCl中进行等密度离心以及在甲基化白蛋白柱上进行分级分离来进行分析。根据DNA在CsCl中的浮力密度ρ，DNA由三类主要DNA组成：一种主体DNA，ρ = 1.699 g/cm³，以及两种卫星DNA，ρ = 1.685 g/cm³和ρ = 1.669 g/cm³。这三种类型的DNA在昆虫的整个发育过程中持续存在。在未受精卵中，总DNA的80%由卫星DNA组成；在受精后的前三小时内，这一比例降至18%，然后在胚胎发生过程中保持恒定。在胚盘形成后，卫星DNA与主体DNA同时增加。
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