Winters Anthony J, Hocart Charles H, Schnitzler Jörg-Peter, Zimmer Ina, Adams Mark A, Rennenberg Heinz, Kreuzwieser Jürgen, Keitel Claudia
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW 2570, Australia.
Research School of Biology, Australian National University, Canberra, ACT 2600, Australia.
Molecules. 2025 May 21;30(10):2234. doi: 10.3390/molecules30102234.
The contributions of de novo synthesis to terpene emissions from subsp. were determined by fumigating branchlets with CO in a gas exchange system. Of more than thirty-four terpenes emitted by this species, only four, i.e., isoprene, iso-valeraldehyde, -ocimene, and trans-caryophyllene, incorporated C into the terpene carbon skeleton during the ~5-6 h experiment. C incorporation into isoprene and iso-valeraldehyde reached a maximum of ca. 82% of the carbon skeleton, similar to -ocimene, with a maximum of 77% C incorporation after ~2.5 h exposure to CO. Only ca. 20% of carbon was labelled in trans-caryophyllene after 5-6 h. the incorporation of C was observed only in compounds emitted from leaves, and was not detected in either individual oil glands or in bulk leaf tissue. The results suggest the de novo synthesis of some terpenes (isoprene, -ocimene, trans-caryophyllene, and iso-valeraldehyde) and their emission is independent of emissions of terpenes stored in oil glands.
通过在气体交换系统中用一氧化碳熏蒸小枝，确定了从头合成对该亚种萜烯排放的贡献。在该物种排放的三十四种以上萜烯中，在约5-6小时的实验期间，只有四种，即异戊二烯、异戊醛、罗勒烯和反式石竹烯，将碳纳入萜烯碳骨架。碳纳入异戊二烯和异戊醛的比例最高达到碳骨架的约82%，与罗勒烯相似，在接触一氧化碳约2.5小时后，碳纳入率最高达到77%。在5-6小时后，反式石竹烯中只有约20%的碳被标记。碳的纳入仅在叶片排放的化合物中观察到，在单个油腺或叶片整体组织中均未检测到。结果表明，一些萜烯（异戊二烯、罗勒烯、反式石竹烯和异戊醛）的从头合成及其排放与储存在油腺中的萜烯排放无关。
