Jamin N, James T L, Zon G
Eur J Biochem. 1985 Oct 1;152(1):157-66. doi: 10.1111/j.1432-1033.1985.tb09176.x.
The resonances of nearly all 70 of the non-exchangeable protons of the duplex [d(GGTATACC)]2 in aqueous solution are assigned by proton two-dimensional nuclear Overhauser enhancement (2D NOE) spectra obtained in pure absorption phase at 500 MHz. Experimental and theoretical 2D NOE spectra are compared at each mixing time (100, 175, 250 and 400 ms) using two B-DNA structures: a standard B-form and an energy-minimized form. The GG and CC ends of the octamer duplex are well represented by the regular B-DNA structure. But large discrepancies from these models are observed for the 'TATA' box. All 2D NOE data are consistent with nanosecond correlation times, as indicated by non-selective proton spin-lattice relaxation times, but small variations in the correlation time are observed, suggesting that there are some local differences in mobility within the octamer duplex structure in solution.
通过在500 MHz下于纯吸收相获得的质子二维核Overhauser增强（2D NOE）光谱，确定了双链体[d(GGTATACC)]₂在水溶液中近70个不可交换质子的共振峰。使用两种B-DNA结构：标准B型和能量最小化形式，在每个混合时间（100、175、250和400毫秒）对实验和理论2D NOE光谱进行比较。八聚体双链体的GG和CC末端由规则的B-DNA结构很好地表示。但在“TATA”框处观察到与这些模型有很大差异。所有2D NOE数据与纳秒级相关时间一致，如非选择性质子自旋-晶格弛豫时间所示，但观察到相关时间有小的变化，这表明溶液中八聚体双链体结构内的流动性存在一些局部差异。
