DNA双螺旋的氢键效应与碳-13核磁共振
Hydrogen-bonding effects and 13C-NMR of the DNA double helix.
作者信息
Borer P N, LaPlante S R, Zanatta N, Levy G C
机构信息
NIH Research Resource, Syracuse University, NY 13244-1200.
出版信息
Nucleic Acids Res. 1988 Mar 25;16(5):2323-32. doi: 10.1093/nar/16.5.2323.
Abstract
13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases.
摘要
DNA中核苷酸的13C-核磁共振化学位移对氢键敏感，尤其是对于直接与作为氢键受体或供体的环外氧或氮原子相连的三个碳原子。在寡脱氧核苷酸双链体中，无论碱基序列如何，当形成沃森-克里克配对时，鸟苷的C2、C6以及胸苷的C4会强烈去屏蔽（约1 ppm）。这些位点的去屏蔽现象可能有助于区分参与沃森-克里克配对的碱基与未配对的碱基。
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