利用核Overhauser效应确定脱氧八聚核苷酸七磷酸d(G-G-C*-C*-G-G-C-C)中不可交换碱基质子和H1'共振峰的归属
Assignment of non-exchangeable base proton and H1' resonances of a deoxyoctanucleoside heptaphosphate d(G-G-C*-C*-G-G-C-C) by using the nuclear Overhauser effect.
作者信息
Sanderson M R, Mellema J R, van der Marel G A, Wille G, van Boom J H, Altona C
出版信息
Nucleic Acids Res. 1983 May 25;11(10):3333-46. doi: 10.1093/nar/11.10.3333.
Abstract
The resonances of the non-exchangeable base protons and 1' protons of the octamer d(G-G-C*-C*-G-G-C-C), C* = m5dC, have been assigned by means of NOE difference NMR spectroscopy at 500 MHz. From the measured J1'2' and J1'2" it follows that the octamer at low temperature prefers to adopt a B-DNA double-helical conformation in solution, however, some residual conformational freedom is detected at the 3' terminus. From the chemical shift versus temperature profiles it is concluded that no major conformational change occurs below 60-65 degrees C where the duplex formation for residues (2) to (6) is essentially completed under the conditions used.
摘要
八聚体d(G-G-C*-C*-G-G-C-C)（C* = m5dC）中不可交换碱基质子和1'质子的共振峰已通过500 MHz的NOE差示核磁共振光谱法进行了归属。根据测得的J1'2'和J1'2"可知，该八聚体在低温下在溶液中倾向于采用B-DNA双螺旋构象，然而，在3'末端检测到一些残留的构象自由度。从化学位移与温度的关系曲线可以得出结论，在60 - 65摄氏度以下没有发生重大的构象变化，在所用条件下，残基(2)至(6)的双链形成基本完成。
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