Anti-Biofilm Properties of Cell-Free Supernatant from EA73 by In Vitro Study with .

Endophytes are a potential source of novel bioactive antimicrobial compounds. A previous study showed that EA73, an endophytic bacterium, has promising antibacterial activity against ; however, the mechanisms associated with its activity have still not been investigated. Therefore, this study was conducted to investigate the molecular mechanisms involved in the anti-biofilm activity of a cell-free supernatant (CFS) of EA73 against . In this experiment, the biofilm-eliminating effects of a CFS of EA73 against were examined in vitro. RT-qPCR was used to detect the changes in genes related to biofilm formation, whereas network pharmacology was used to predict the key targets and pathways of a cell-free supernatant of EA73 against -mediated diseases. The minimum biofilm eradication concentration (MBEC) of the EA73 CFS against was 1.28 × 10 g/mL. In addition, we observed that the EA73 CFS reduced bacterial adhesion and decreased extracellular proteins, polysaccharides, and the eDNA content in the biofilm and decreased the expression of biofilm-associated genes, such as and . The EA73 CFS had a significant effect on biofilm eradication and hence can serve as a promising antibacterial reagent.