• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自EA73的无细胞上清液的抗生物膜特性的体外研究

Anti-Biofilm Properties of Cell-Free Supernatant from EA73 by In Vitro Study with .

作者信息

Tang Ziyao, Yousif Muhammad, Okyere Samuel Kumi, Liao Fei, Peng Siqi, Cheng Lin, Yang Feng, Wang Yuting, Hu Yanchun

机构信息

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

Department of Veterinary Surgery & Obstetrics, Faculty of Veterinary Sciences, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences (SBBUVAS), Sakrand 67210, Pakistan.

出版信息

Microorganisms. 2025 May 20;13(5):1162. doi: 10.3390/microorganisms13051162.

DOI:10.3390/microorganisms13051162
PMID:40431333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12114590/
Abstract

Endophytes are a potential source of novel bioactive antimicrobial compounds. A previous study showed that EA73, an endophytic bacterium, has promising antibacterial activity against ; however, the mechanisms associated with its activity have still not been investigated. Therefore, this study was conducted to investigate the molecular mechanisms involved in the anti-biofilm activity of a cell-free supernatant (CFS) of EA73 against . In this experiment, the biofilm-eliminating effects of a CFS of EA73 against were examined in vitro. RT-qPCR was used to detect the changes in genes related to biofilm formation, whereas network pharmacology was used to predict the key targets and pathways of a cell-free supernatant of EA73 against -mediated diseases. The minimum biofilm eradication concentration (MBEC) of the EA73 CFS against was 1.28 × 10 g/mL. In addition, we observed that the EA73 CFS reduced bacterial adhesion and decreased extracellular proteins, polysaccharides, and the eDNA content in the biofilm and decreased the expression of biofilm-associated genes, such as and . The EA73 CFS had a significant effect on biofilm eradication and hence can serve as a promising antibacterial reagent.

摘要

内生菌是新型生物活性抗菌化合物的潜在来源。先前的一项研究表明，内生细菌EA73对[具体对象未明确]具有有前景的抗菌活性；然而，其活性相关机制仍未得到研究。因此，本研究旨在探究EA73无细胞上清液（CFS）对[具体对象未明确]的抗生物膜活性所涉及的分子机制。在本实验中，体外检测了EA73的CFS对[具体对象未明确]的生物膜消除作用。RT-qPCR用于检测与生物膜形成相关基因的变化，而网络药理学用于预测EA73无细胞上清液对[具体对象未明确]介导疾病的关键靶点和途径。EA73 CFS对[具体对象未明确]的最低生物膜根除浓度（MBEC）为1.28×10 g/mL。此外，我们观察到EA73 CFS降低了细菌粘附，减少了生物膜中的细胞外蛋白质、多糖和胞外DNA含量，并降低了生物膜相关基因如[具体基因未明确]和[具体基因未明确]的表达。EA73 CFS对[具体对象未明确]生物膜根除具有显著作用，因此可作为一种有前景的抗菌试剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/a2f0f140642f/microorganisms-13-01162-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/1b0519c16012/microorganisms-13-01162-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/bcf8728daaf2/microorganisms-13-01162-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/dc1bc3e014cc/microorganisms-13-01162-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/7a641f81e6ed/microorganisms-13-01162-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/16ee379f1d06/microorganisms-13-01162-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/0be9e15f5ce1/microorganisms-13-01162-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/a736b6bf6295/microorganisms-13-01162-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/66c45a5653ca/microorganisms-13-01162-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/998646192d3d/microorganisms-13-01162-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/4df1b8256e3b/microorganisms-13-01162-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/8312f28cb266/microorganisms-13-01162-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/6b031a9a08a6/microorganisms-13-01162-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/4983725cf197/microorganisms-13-01162-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/a2f0f140642f/microorganisms-13-01162-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/1b0519c16012/microorganisms-13-01162-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/bcf8728daaf2/microorganisms-13-01162-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/dc1bc3e014cc/microorganisms-13-01162-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/7a641f81e6ed/microorganisms-13-01162-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/16ee379f1d06/microorganisms-13-01162-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/0be9e15f5ce1/microorganisms-13-01162-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/a736b6bf6295/microorganisms-13-01162-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/66c45a5653ca/microorganisms-13-01162-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/998646192d3d/microorganisms-13-01162-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/4df1b8256e3b/microorganisms-13-01162-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/8312f28cb266/microorganisms-13-01162-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/6b031a9a08a6/microorganisms-13-01162-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/4983725cf197/microorganisms-13-01162-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/563f/12114590/a2f0f140642f/microorganisms-13-01162-g014.jpg

相似文献

1
Anti-Biofilm Properties of Cell-Free Supernatant from EA73 by In Vitro Study with .来自EA73的无细胞上清液的抗生物膜特性的体外研究
Microorganisms. 2025 May 20;13(5):1162. doi: 10.3390/microorganisms13051162.
2
Antibacterial Activity of Two Metabolites Isolated From Endophytic Bacteria Ea73 in .从内生细菌Ea73中分离出的两种代谢产物的抗菌活性 于……
Front Microbiol. 2022 May 6;13:860009. doi: 10.3389/fmicb.2022.860009. eCollection 2022.
3
Inhibitory effect of Bacillus velezensis 1273 strain cell-free supernatant against developing and preformed biofilms of Staphylococcus aureus and MRSA.解淀粉芽孢杆菌 1273 菌株无细胞上清液对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌生物膜形成和发展的抑制作用。
Microb Pathog. 2024 Dec;197:107065. doi: 10.1016/j.micpath.2024.107065. Epub 2024 Oct 22.
4
Cell-Free Supernatant of Bacillus thuringiensis Displays Anti-Biofilm Activity Against Staphylococcus aureus.苏云金芽孢杆菌无细胞上清液对金黄色葡萄球菌表现出抗生物膜活性。
Appl Biochem Biotechnol. 2023 Sep;195(9):5379-5393. doi: 10.1007/s12010-022-03971-z. Epub 2022 May 20.
5
Anti-Biofilm Activity of Cell-Free Supernatant of against .对 的无细胞上清液的抗生物膜活性。
J Microbiol Biotechnol. 2020 Dec 28;30(12):1854-1861. doi: 10.4014/jmb.2008.08053.
6
Anti-Biofilm Potential of and Cell-Free Supernatant Extracts against .[具体物质]和无细胞上清液提取物对[具体细菌]的抗生物膜潜力
Adv Biomed Res. 2023 Feb 25;12:50. doi: 10.4103/abr.abr_156_21. eCollection 2023.
7
Impact of cell-free supernatant of on biofilm and its metabolites.[物质名称]的无细胞上清液对[生物名称]生物膜及其代谢产物的影响。
Front Vet Sci. 2023 Jun 15;10:1184989. doi: 10.3389/fvets.2023.1184989. eCollection 2023.
8
AP183 Inhibits Biofilm Formation and Proliferation in Murine and Bovine Disease Models.AP183在小鼠和牛疾病模型中抑制生物膜形成和增殖。
Front Microbiol. 2021 Oct 8;12:746410. doi: 10.3389/fmicb.2021.746410. eCollection 2021.
9
Exopolysaccharides produced by Bacillus spp. inhibit biofilm formation by Staphylococcus aureus strains associated with bovine mastitis.芽孢杆菌属产生的胞外多糖可抑制与牛乳腺炎相关的金黄色葡萄球菌菌株形成生物膜。
Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126689. doi: 10.1016/j.ijbiomac.2023.126689. Epub 2023 Sep 9.
10
Investigating the antimicrobial and antibiofilm properties of marine halophilic Bacillus species against ESKAPE pathogens.研究海洋嗜盐芽孢杆菌对 ESKAPE 病原体的抗菌和抗生物膜特性。
Environ Microbiol Rep. 2024 Oct;16(5):e70027. doi: 10.1111/1758-2229.70027.

本文引用的文献

1
Inhibitory effect of Bacillus velezensis 1273 strain cell-free supernatant against developing and preformed biofilms of Staphylococcus aureus and MRSA.解淀粉芽孢杆菌 1273 菌株无细胞上清液对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌生物膜形成和发展的抑制作用。
Microb Pathog. 2024 Dec;197:107065. doi: 10.1016/j.micpath.2024.107065. Epub 2024 Oct 22.
2
Biosynthesis of silver nanoparticle using Bacillus licheniformis culture-supernatant for combating pathogenic biofilms.利用地衣芽孢杆菌培养上清液合成银纳米颗粒以对抗致病生物膜。
Microb Pathog. 2024 Sep;194:106833. doi: 10.1016/j.micpath.2024.106833. Epub 2024 Aug 5.
3
Gallic acid exerts antibiofilm activity by inhibiting methicillin-resistant Staphylococcus aureus adhesion.
没食子酸通过抑制耐甲氧西林金黄色葡萄球菌黏附来发挥抗生物膜活性。
Sci Rep. 2024 Jul 26;14(1):17220. doi: 10.1038/s41598-024-68279-w.
4
The wound microbiota: microbial mechanisms of impaired wound healing and infection.伤口菌群：影响伤口愈合和感染的微生物机制。
Nat Rev Microbiol. 2024 Aug;22(8):507-521. doi: 10.1038/s41579-024-01035-z. Epub 2024 Apr 4.
5
Involvement of PAR-2 in the Induction of Cell-Specific Matrix Metalloproteinase-2 by Activated Protein C in Cutaneous Wound Healing.蛋白水解激活受体-2 在活化蛋白 C 诱导皮肤创伤愈合中细胞特异性基质金属蛋白酶-2 表达中的作用
Int J Mol Sci. 2023 Dec 27;25(1):370. doi: 10.3390/ijms25010370.
6
Adaptive evolution of the Clf-Sdr subfamily contributes to Staphylococcus aureus musculoskeletal infection: Evidence from comparative genomics.Clf-Sdr 亚家族的适应性进化有助于金黄色葡萄球菌肌肉骨骼感染：来自比较基因组学的证据。
Microbiol Res. 2024 Jan;278:127502. doi: 10.1016/j.micres.2023.127502. Epub 2023 Sep 17.
7
A novel indolylbenzoquinone compound HL-J6 suppresses biofilm formation and α-toxin secretion in methicillin-resistant Staphylococcus aureus.一种新型吲哚并苯醌化合物 HL-J6 抑制耐甲氧西林金黄色葡萄球菌生物膜形成和α-毒素分泌。
Int J Antimicrob Agents. 2023 Nov;62(5):106972. doi: 10.1016/j.ijantimicag.2023.106972. Epub 2023 Sep 21.
8
Network pharmacology- and molecular docking-based analyses of the antihypertensive mechanism of .基于网络药理学和分子对接的. 降压机制分析。
Front Endocrinol (Lausanne). 2023 Aug 17;14:1216086. doi: 10.3389/fendo.2023.1216086. eCollection 2023.
9
Alpha-amyrin as an anti-biofilm agent against methicillin-resistant and vancomycin-intermediate s.齐墩果酸作为一种抗生物膜剂，用于对抗耐甲氧西林和万古霉素中介的[细菌名称未完整给出]。
Heliyon. 2023 Jul 5;9(7):e17892. doi: 10.1016/j.heliyon.2023.e17892. eCollection 2023 Jul.
10
Small-Molecule Compound CY-158-11 Inhibits Staphylococcus aureus Biofilm Formation.小分子化合物 CY-158-11 抑制金黄色葡萄球菌生物膜形成。
Microbiol Spectr. 2023 Jun 15;11(3):e0004523. doi: 10.1128/spectrum.00045-23. Epub 2023 May 11.