Quitschke W, Sibony P, Schechter N
Exp Neurol. 1985 Oct;90(1):204-14. doi: 10.1016/0014-4886(85)90053-6.
The intermediate filament protein composition of human optic nerve and tract was analyzed by two-dimensional gel electrophoresis. Optic nerves were analyzed at 15, 19, 22, and 24 weeks of embryonic development and the results were compared with the composition of adult optic nerve. The optic tract was analyzed at the later stages of development. The proteins were visualized by Coomassie blue staining and immunoblotting with specific antibodies to glial fibrillary acidic protein (GFAP) and vimentin. The 70K and 150K neurofilament proteins were first observed at 24 weeks. At 15 weeks of development, only trace amounts of GFAP were observed and vimentin was the predominant intermediate filament protein. In contrast, there was more than twice as much GFAP as vimentin in the adult optic nerve. The results also showed that the ratio of GFAP to vimentin is higher in the optic tract than in the optic nerve during development, whereas in adult tissue, the ratio is the same for the two regions. In pathological situations with axonal dropout, a complete loss of neurofilament proteins was observed. The amounts of both GFAP and vimentin were the same for both the normal and involved optic nerve. These results are discussed in terms of the development of the optic nerve.
采用二维凝胶电泳分析了人类视神经和视束的中间丝蛋白组成。在胚胎发育的第15、19、22和24周对视神经进行分析，并将结果与成人视神经的组成进行比较。在发育后期对视束进行分析。通过考马斯亮蓝染色以及用针对胶质纤维酸性蛋白（GFAP）和波形蛋白的特异性抗体进行免疫印迹来观察蛋白质。70K和150K神经丝蛋白在24周时首次被观察到。在发育的第15周，仅观察到微量的GFAP，波形蛋白是主要的中间丝蛋白。相比之下，成人视神经中GFAP的含量是波形蛋白的两倍多。结果还表明，在发育过程中，视束中GFAP与波形蛋白的比例高于视神经，而在成体组织中，这两个区域的比例相同。在轴突缺失的病理情况下，观察到神经丝蛋白完全缺失。正常和受累视神经中GFAP和波形蛋白的含量相同。根据视神经的发育情况对这些结果进行了讨论。
