Bakkeren D L, de Jeu-Jaspars C M, van der Heul C, van Eijk H G
Int J Biochem. 1985;17(8):925-30. doi: 10.1016/0020-711x(85)90177-6.
Rat reticulocytes were incubated with rat 125I-Tf-59Fe under conditions inhibiting heme synthesis. Cytosol, prepared from the reticulocytes, was separated and analysed by gel filtration and Amicon Ultrafiltration. An iron-containing low molecular weight fraction derived from the cytosol was further analysed by HPLC size-exclusion chromatography and HPLC reversed phase chromatography. Conditions inhibiting heme synthesis and uncoupling the oxidative phosphorylations lead to a large increase in the Fe-containing low molecular weight fraction in the cytosol. The components in the low molecular weight fraction have an apparent molecular weight of 5500 Dalton as determined with HPLC size-exclusion chromatography. The low molecular weight fraction contained several iron chelating components like glycin, 1/2 cystine and citrate, but no specific iron-binding proteins, nucleotides or pyrophosphate.
在抑制血红素合成的条件下，将大鼠网织红细胞与大鼠125I-转铁蛋白-59Fe一起孵育。从网织红细胞制备细胞溶质，通过凝胶过滤和密理博超滤进行分离和分析。通过高效液相色谱尺寸排阻色谱法和高效液相色谱反相色谱法进一步分析源自细胞溶质的含铁低分子量组分。抑制血红素合成并使氧化磷酸化解偶联的条件导致细胞溶质中含铁低分子量组分大幅增加。通过高效液相色谱尺寸排阻色谱法测定，低分子量组分中的成分表观分子量为5500道尔顿。低分子量组分包含几种铁螯合成分，如甘氨酸、1/2胱氨酸和柠檬酸盐，但没有特异性铁结合蛋白、核苷酸或焦磷酸盐。
