从豚鼠网织红细胞中分离出的低分子量铁以AMP-铁和ATP-铁复合物的形式存在。
Low-Mr iron isolated from guinea pig reticulocytes as AMP-Fe and ATP-Fe complexes.
作者信息
Weaver J, Pollack S
机构信息
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
出版信息
Biochem J. 1989 Aug 1;261(3):787-92. doi: 10.1042/bj2610787.
Abstract
Guinea pig reticulocytes were pulse-labelled with 59Fe bound to transferrin. Haemolysates prepared from these reticulocytes were subjected to rapid (NH1)2SO1 precipitation and then chromatography on an anion-exchange resin. ATP-bound 59Fe was the dominant species in the reticulocyte cytosol; 2,3-bisphosphoglycerate and GTP iron complexes were not detected despite the fact that these were stable with (NH1)2SO1 precipitation and readily detected with anion-exchange chromatography. AMP-bound Fe was a minor component of the cytosol following rapid (NH1)2SO4 precipitation, and the major component when iron was released from transferrin by haemolysates. We speculate that ATP-Fe may be degraded in the cell to permit utilization of its iron for haem synthesis.
摘要
豚鼠网织红细胞用与转铁蛋白结合的59Fe进行脉冲标记。从这些网织红细胞制备的溶血产物先进行快速硫酸铵沉淀，然后在阴离子交换树脂上进行色谱分析。与ATP结合的59Fe是网织红细胞胞质溶胶中的主要成分；尽管2,3-二磷酸甘油酸和GTP铁复合物在硫酸铵沉淀中稳定且通过阴离子交换色谱易于检测，但未检测到。快速硫酸铵沉淀后，与AMP结合的铁是胞质溶胶中的次要成分，而当溶血产物使铁从转铁蛋白中释放时，它是主要成分。我们推测，ATP-铁可能在细胞内被降解，以便利用其铁进行血红素合成。
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