Kuryltsiv Nadiia
Department of Ophthalmology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
Korean J Ophthalmol. 2025 Jun;39(3):258-268. doi: 10.3341/kjo.2024.0118. Epub 2025 May 28.
To study and compare the immune response and neopterin levels in the blood in experimental autoimmune uveitis (EAU).
A model of EAU was created in 30 Chinchilla rabbits. Intravenous and intravitreal injections of normal horse serum were administered for this purpose. Clinical examinations and blood tests were conducted on days 3, 7, 10, 14, and 21. The blood investigation included the determination of neopterin (NP) level, white blood cell counts, lymphocytes, CD3+, CD4+, CD8+, and CD16+.
The peak in white blood cell count was observed on days 7 and 10 (6.4 ± 0.4 g/L and 6.0 ± 0.3 g/L, respectively), lymphocytes on day 3 (68.3% ± 2.4%, 3.0 ± 0.2 g/L), CD3+ on day 7 (64.9% ± 3.1%, 2,032.5 ± 91.2 cells/µL), CD4+ and CD16+ on day 10 (54.6% ± 3.8%, 2,462.3 ± 60.7 cells/µL and 21.8% ± 1.8%, 691.2 ± 37.1 cells/µL, respectively). All these values did not return to the initial ones. There was a gradual decrease in the CD8+ count from day 3 (12.5% ± 1.1%, 142.8 ± 9.1 cells/µL) with a subsequent gradual return towards normal levels by day 21. NP levels incresed on day 3 (5.2 ± 0.7 nmol/L), sustained on day 7 (5.2 ± 0.8 nmol/L), and started to decrease from day 10 (4.25 ± 1.7 nmol/L) to 2.3 ± 0.5 nmol/L on day 21. The highest correlation was observed between clinical manifestations and NP with a correlation coeffient of 0.799 (95% confidence interval, 0.719-0.858), which was significantly stronger (p < 0.05) than the correlations with other immune response markers.
During the modeling of EAU, there is an active immune response and a rapid reaction of NP on inflammation. NP is a significantly more sensitive marker of intraocular inflammation than the immune response. It can serve as a predictor of the onset and development of EAU.
研究并比较实验性自身免疫性葡萄膜炎（EAU）中血液的免疫反应及新蝶呤水平。
在30只青紫蓝兔中建立EAU模型。为此进行静脉及玻璃体内注射正常马血清。在第3、7、10、14和21天进行临床检查及血液检测。血液检测包括测定新蝶呤（NP）水平、白细胞计数、淋巴细胞、CD3⁺、CD4⁺、CD8⁺及CD16⁺。
白细胞计数在第7天和第10天出现峰值（分别为6.4±0.4 g/L和6.0±0.3 g/L），淋巴细胞在第3天（68.3%±2.4%，3.0±0.2 g/L），CD3⁺在第7天（64.9%±3.1%，2,032.5±91.2个细胞/µL），CD4⁺和CD16⁺在第10天（分别为54.6%±3.8%，2,462.3±60.7个细胞/µL和21.8%±1.8%，691.2±37.1个细胞/µL）。所有这些值均未恢复至初始值。CD8⁺计数从第3天（12.5%±1.1%，142.8±9.1个细胞/µL）开始逐渐下降，到第21天随后逐渐恢复至正常水平。NP水平在第3天升高（5.2±0.7 nmol/L），第7天维持（5.2±0.8 nmol/L），并从第10天开始下降（4.25±1.7 nmol/L），至第21天降至2.3±0.5 nmol/L。在临床表现与NP之间观察到最高相关性，相关系数为0.799（95%置信区间，0.719 - 0.858），这比与其他免疫反应标志物的相关性显著更强（p < 0.05）。
在EAU建模过程中，存在活跃的免疫反应以及NP对炎症的快速反应。NP是比免疫反应更敏感的眼内炎症标志物。它可作为EAU发病及发展的预测指标。
