Mozaffari Elaheh, Mehrinejad Khotbehsara Maryam, Faghani Mostafa, Asadi Shiva, Hoseinzadeh Saghar, Allahyartorkaman Mohammadreza, Nemati Reza, Salimipour Houman, Fazilat Ahmad
Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
School of Business, University of Southern Queensland, Toowoomba, Australia.
Neurol Res. 2025 Sep;47(9):807-816. doi: 10.1080/01616412.2025.2504146. Epub 2025 May 28.
Migraine is a complex neurological disorder lacking reliable assessment methods. Non-steroidal anti-inflammatory drugs relieve migraine pain by inhibiting prostaglandin synthesis through COX-1 and COX-2 suppression. As COX-2 plays a key role in pain and inflammation, its modulation is vital in migraine therapy. We hypothesized that the COX-2 8473 T>C (rs5275) gene variant may be linked to migraine, depression, and nausea.
In this case-control study, genomic DNA from 100 migraine patients and 100 controls was analyzed for the polymorphism using PCR-RFLP.
Statistical analysis revealed a significant association between the variant and increased risk of migraine progression, depression, and nausea. The T+ genotype was more prevalent in controls than in patients (93% and 68% respectively; < 0.0001), while the C+ genotype was more frequent in patients than controls (70% and 25% respectively; < 0.001). Among migraineurs with depression, 75% carried the C+ genotype compared to 25% in controls ( < 0.001). Similarly, 76.1% of migraine patients with nausea had the C+ genotype, versus 25% of controls ( < 0.001).
The genotype appears to increase the risk of migraine, depression, and nausea, while the T+ genotype may have a protective effect. This comparative genomics study highlights the potential role of the genotype in migraine manifestation, though further research is needed to clarify its pathogenic involvement.
偏头痛是一种缺乏可靠评估方法的复杂神经系统疾病。非甾体抗炎药通过抑制环氧化酶-1（COX-1）和环氧化酶-2（COX-2）来抑制前列腺素合成，从而缓解偏头痛疼痛。由于COX-2在疼痛和炎症中起关键作用，其调节在偏头痛治疗中至关重要。我们推测COX-2 8473 T>C（rs5275）基因变异可能与偏头痛、抑郁和恶心有关。
在这项病例对照研究中，使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析了100例偏头痛患者和100例对照的基因组DNA中的多态性。
统计分析显示该变异与偏头痛进展、抑郁和恶心风险增加之间存在显著关联。T+基因型在对照组中比患者中更常见（分别为93%和68%；<0.0001），而C+基因型在患者中比对照组更频繁（分别为70%和25%；<0.001）。在伴有抑郁的偏头痛患者中，75%携带C+基因型，而对照组为25%（<0.001）。同样，76.1%有恶心症状的偏头痛患者具有C+基因型，而对照组为25%（<0.001）。
该基因型似乎会增加偏头痛、抑郁和恶心的风险，而T+基因型可能具有保护作用。这项比较基因组学研究突出了该基因型在偏头痛表现中的潜在作用，不过还需要进一步研究来阐明其致病机制。
