Krawczyk Katarzyna, Maździarz Mateusz, Paukszto Łukasz, Nobis Marcin, Sawicki Jakub
Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn, warmińsko-mazurskie, 10-727, Poland.
Faculty of Biology, Institute of Botany, Jagiellonian University, Gronostajowa 3A, Kraków, małopolskie, 30-387, Poland.
Cladistics. 2025 Aug;41(4):358-371. doi: 10.1111/cla.12618. Epub 2025 May 28.
Compared to plastid genomes, plant mitochondrial genomes have been less frequently used for species discrimination and phylogenetic studies due to assembly challenges, lower substitution rates and rapid structural evolution. However, this study demonstrates that mitochondrial genome fragments can be valuable for both molecular species identification and phylogenetic analysis in grasses of the tribe Stipeae. To explore this potential, we first assembled the complete mitochondrial genome of Nassella tenuissima-the first fully described mitogenome in Stipeae-which served as a reference for selecting 29 aligned mitochondrial genome fragments totalling 139 680 bp. These fragments were then analysed across 49 representatives of the tribe, including 43 Stipa species and six other taxa. The mitochondrial fragments achieved a species discrimination efficiency of 75%, slightly exceeding the 71% efficiency observed for plastid super-barcodes. Additionally, comparative phylogenetic analyses using plastid and mitochondrial genomes underscored the utility of mitochondrial data in resolving phylogenetic relationships within Stipeae. Our findings provide a valuable resource for future research in transcriptomics, comparative genomics, phylogenomics and phylogeography of grasses.
与质体基因组相比，由于组装挑战、较低的替换率和快速的结构进化，植物线粒体基因组在物种鉴别和系统发育研究中的应用频率较低。然而，本研究表明，线粒体基因组片段对于针茅族禾本科植物的分子物种鉴定和系统发育分析都具有重要价值。为了探索这种潜力，我们首先组装了细茎针茅的完整线粒体基因组——这是针茅族中第一个完全描述的有丝分裂基因组——作为选择29个比对的线粒体基因组片段的参考，这些片段总长139680bp。然后对该族的49个代表物种进行了分析，包括43个针茅属物种和其他6个分类群。线粒体片段的物种鉴别效率达到了75%，略高于质体超级条形码的71%的效率。此外，使用质体和线粒体基因组进行的比较系统发育分析强调了线粒体数据在解决针茅族内系统发育关系方面的实用性。我们的研究结果为禾本科植物转录组学、比较基因组学、系统发育基因组学和系统地理学的未来研究提供了宝贵资源。
