Yamazaki S, Machii K, Tsuyuki S, Momose H, Kawashima T, Ueda K
Immunology. 1985 Sep;56(1):43-50.
After monoassociation of Bifidobacterium longum with germ-free BALB/c mice (nu/+, heterozygous to nu gene), B. longum was recovered (10(2)-10(4) viable units per organ) from the mesenteric lymph nodes, liver and kidneys for 2 weeks post intragastric inoculation, but not after 4 weeks and later. Cessation of bacterial translocation was not observed in monoassociated nude (nu/nu) mice. Anti-B. longum IgA antibody was detected by ELISA using B. longum cell wall antigen in bile from Week 1 and in ileac wall extract from Week 8 post-association in both nu/+ and nu/nu mice. Total IgA levels in bile, ileac wall extract and caecal contents were also elevated in both mice after monoassociation. Cell-mediated immunity measured by the footpad test and macrophage migration inhibition test using B. longum protein fraction was detected in nu/+ mice in Week 4 and later, but not in nu/nu mice. Nu/nu mice reconstituted 4 weeks prior to monoassociation with lymphocytes from flora bearing nu/+ mice developed delayed footpad reactivity and bacterial translocation stopped after 4 weeks. Cell-mediated immunity rather than IgA antibody correlated well with the cessation of translocation.
将长双歧杆菌单联接种到无菌BALB/c小鼠（nu/+，nu基因杂合子）体内后，在胃内接种后2周，可从肠系膜淋巴结、肝脏和肾脏中回收到长双歧杆菌（每个器官有10(2)-10(4)个活单位），但在4周及以后则无法回收。在单联的裸鼠（nu/nu）中未观察到细菌易位的停止。在nu/+和nu/nu小鼠中，从单联后第1周的胆汁和第8周的回肠壁提取物中，使用长双歧杆菌细胞壁抗原通过ELISA检测到抗长双歧杆菌IgA抗体。单联后，两种小鼠的胆汁、回肠壁提取物和盲肠内容物中的总IgA水平也都升高。在第4周及以后，在nu/+小鼠中通过使用长双歧杆菌蛋白组分的足垫试验和巨噬细胞迁移抑制试验检测到细胞介导的免疫，但在nu/nu小鼠中未检测到。在单联前4周用来自携带nu/+小鼠菌群的淋巴细胞重建的nu/nu小鼠出现延迟的足垫反应性，并且4周后细菌易位停止。细胞介导的免疫而非IgA抗体与易位的停止密切相关。
