Duffy L C, Zielezny M A, Riepenhoff-Talty M, Dryja D, Sayahtaheri-Altaie S, Griffiths E, Ruffin D, Barrett H, Ogra P L
Department of Pediatrics, Children's Hospital, Buffalo, NY 14222.
Dig Dis Sci. 1994 Nov;39(11):2334-40. doi: 10.1007/BF02087647.
The protective effect of a human strain of Bifidobacterium bifidum (B. bifidum) against murine group A rotavirus (MRV) was examined in the intestines of BALB/c infected mice. In experiments designed to determine whether B. bifidum mediated MRV shedding during diarrheal disease, pregnant dams (and their expected litters) were randomly assigned to the following groups: (1) mice infected with MRV alone; (2) B. bifidum-treated + MRV-infected mice; (3) B. bifidum-treated controls; and (4) saline control animals. An enzyme-linked immunosorbent assay (ELISA) for the detection of group A rotavirus was used to measure virus protein. The sensitivity of the MRV antigen detector ELISA was determined by serially diluting the rotavirus antigen in test samples. Antigen was detected in dilution ranges of 1:256-1:4096 during the acute phase and 1:16-1:512 in the recovery phase of MRV clinical disease, in the samples tested. Treatment with B. bifidum significantly reduced shedding of MRV antigen (P < 0.009) on days 2-10 postinoculation. The reduction in shedding of virus protein corresponded well with delayed onset of acute diarrhea (P < 0.02). Closer examination of tissue cross sections under electron microscopy revealed that the B. bifidum-ingested strain adhered to the epithelium of the small intestine. These results suggest that priming the intestine with B. bifidum is effective against experimental MRV challenge and confirmed the potential usefulness of this detector ELISA for studying the kinetics of group A rotavirus infection in animals and humans.
在感染的BALB/c小鼠肠道中检测了一株人源双歧双歧杆菌（B. bifidum）对鼠A组轮状病毒（MRV）的保护作用。在旨在确定双歧双歧杆菌是否介导腹泻疾病期间MRV排出的实验中，将怀孕的母鼠（及其预期的幼崽）随机分为以下几组：（1）仅感染MRV的小鼠；（2）经双歧双歧杆菌处理 + 感染MRV的小鼠；（3）经双歧双歧杆菌处理的对照小鼠；（4）生理盐水对照动物。使用用于检测A组轮状病毒的酶联免疫吸附测定（ELISA）来测量病毒蛋白。通过在测试样品中连续稀释轮状病毒抗原确定MRV抗原检测ELISA的灵敏度。在所测试的样品中，在MRV临床疾病的急性期，抗原在1:256 - 1:4096的稀释范围内被检测到，在恢复期为1:16 - 1:512。接种后第2 - 10天，用双歧双歧杆菌治疗显著减少了MRV抗原的排出（P < 0.009）。病毒蛋白排出的减少与急性腹泻发作的延迟密切相关（P < 0.02）。在电子显微镜下对组织切片进行更仔细的检查发现，摄入的双歧双歧杆菌菌株粘附在小肠上皮上。这些结果表明，用双歧双歧杆菌预处理肠道对实验性MRV攻击有效，并证实了这种检测ELISA在研究动物和人类A组轮状病毒感染动力学方面的潜在用途。
