Feng Ningning, Xu Ke, Wang Guozhen, Zeng Yihai, Zeng Renzhi, Liang Xin, Wu Haiyan, Liu Hongwei, Xie Jianping
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China.
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China.
Food Chem. 2025 Oct 1;488:144817. doi: 10.1016/j.foodchem.2025.144817. Epub 2025 May 22.
Nitrite, a common food additive and environmental pollutant, poses potential health risks, making its accurate detection essential for food safety. This study reports a nanozyme platform based on modified jute loaded with platinum nanoparticles (Pt/MJT) for the colorimetric detection of nitrite. The Pt/MJT nanozyme exhibits exceptional catalytic activity and selectivity. The catalytic reaction kinetics of this nanozyme conforms to the Michaelis-Menten model, with a low Michaelis constant (Km = 0.39 mM) and a high maximum reaction rate (Vmax = 23.57 × 10^-7 M/s), outperforming many previously reported nanozyme materials. The linear detection range is 10-300 μM, with a limit of detection (LOD) of 0.71 μM. Importantly, the practical applicability of this platform for nitrite detection in food matrices, including sausages and kimchi, has been successfully demonstrated, showing satisfactory recovery and precision. This work provides a novel approach for rapid, on-site nitrite detection in the food industry.
亚硝酸盐是一种常见的食品添加剂和环境污染物，存在潜在健康风险，因此对其进行准确检测对食品安全至关重要。本研究报告了一种基于负载铂纳米颗粒的改性黄麻（Pt/MJT）的纳米酶平台，用于比色法检测亚硝酸盐。Pt/MJT纳米酶具有出色的催化活性和选择性。该纳米酶的催化反应动力学符合米氏模型，米氏常数低（Km = 0.39 mM），最大反应速率高（Vmax = 23.57×10^-7 M/s），优于许多先前报道的纳米酶材料。线性检测范围为10 - 300 μM，检测限（LOD）为0.71 μM。重要的是，该平台在包括香肠和泡菜在内的食品基质中检测亚硝酸盐的实际适用性已得到成功证明，回收率和精密度令人满意。这项工作为食品工业中亚硝酸盐的快速现场检测提供了一种新方法。
