Chang Panfeng, Wang Hua
Animal Science Research Laboratory, Gansu Animal Science and Veterinary Medicine Institute, Ping Liang, Gansu, China.
Anim Reprod. 2025 May 26;22(2):e20240139. doi: 10.1590/1984-3143-AR2024-0139. eCollection 2025.
Intensive semen collection often leads to "kidney yang deficiency" in male yaks, with symptoms like lethargy and poor semen quality. Our modified "Yijing Fang" was tested. Eight 3 - 6-year-old male yaks with this condition were split into treatment and negative control groups; four healthy yaks were a blank control. The treated yaks got the formula in feed for 5 days, then a 20-day break, repeating 3 times over 65 days. Semen was collected, and cAMP, cGMP, T/E2 levels, and mitochondrial apoptosis factors were analyzed. Results showed the formula boosted semen quality. cAMP and testosterone rose (p < 0.01), cGMP and estradiol fell (p < 0.01), cAMP/cGMP and T/E2 ratios climbed (p < 0.01). Bcl-2 upregulated, Bax, Cyt-C, and Caspase-3 downregulated (p < 0.01). In conclusion, the modified "Yijing Fang" is effective for yak reproduction, enhancing hormone secretion and reducing sperm apoptosis. Long-term studies are needed.
频繁采精常导致雄性牦牛出现“肾阳虚”，表现为嗜睡和精液品质不佳等症状。我们对改良后的“益精方”进行了测试。将八头患有此症状的3至6岁雄性牦牛分为治疗组和阴性对照组；四头健康牦牛作为空白对照组。对治疗组牦牛在饲料中添加该方剂，持续5天，随后休息20天，在65天内重复3次。采集精液，并分析cAMP、cGMP、T/E2水平以及线粒体凋亡因子。结果表明该方剂提高了精液品质。cAMP和睾酮升高（p < 0.01），cGMP和雌二醇降低（p < 0.01），cAMP/cGMP和T/E2比值升高（p < 0.01）。Bcl-2上调，Bax、Cyt-C和Caspase-3下调（p < 0.01）。总之，改良后的“益精方”对牦牛繁殖有效，可增强激素分泌并减少精子凋亡。尚需进行长期研究。
