DpWRI1 样基因过表达对转基因微小杜氏藻的影响。
Effects of DpWRI1-like gene overexpression in transgenic Dunaliella parva.
作者信息
Ruan Lingru, Lin Juying, Wu Lina, Liang Yanyan, Zhang Xiumin, Liang Shiyu, Huang Limei, Shang Changhua
机构信息
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education and Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) and University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guilin, 541006, Guangxi, China.
出版信息
Antonie Van Leeuwenhoek. 2025 May 30;118(7):84. doi: 10.1007/s10482-025-02095-8.
WRINKLED1 (WRI1) is a transcription factor (TF) of the APETALA2 (AP2) family, which regulates oil synthesis in plants. Dunaliella parva is a microalga, which attracts widespread attention due to its strong resistance to unfavorable environments and simple cultivation conditions. In our earlier study, DpWRI1-like was identified but the role of WRI1 for oil accumulation remains unclear in D. parva. Therefore, in this study, the effects of DpWRI1-like overexpression on D. parva were evaluated. Overexpressing DpWRI1-like could greatly increase the amount of oil while decreasing the amount of starch in transgenic D. parva. In addition, mRNA levels of many genes (ACC, GPAT and DGAT) related to fatty acid synthesis/triacylglycerol (TAG) synthesis increased in transgenic D. parva. In conclusion, the DpWRI1-like protein could convert carbon flow to the biosynthesis of fatty acids and triacylglycerol (TAG).
皱叶1（WRI1）是APETALA2（AP2）家族的转录因子（TF），可调节植物中的油脂合成。杜氏盐藻是一种微藻，因其对不利环境的强抗性和简单的培养条件而受到广泛关注。在我们早期的研究中，鉴定出了类DpWRI1，但WRI1在杜氏盐藻中对油脂积累的作用仍不清楚。因此，在本研究中，评估了类DpWRI1过表达对杜氏盐藻的影响。过表达类DpWRI1可显著增加转基因杜氏盐藻中的油脂含量，同时降低淀粉含量。此外，转基因杜氏盐藻中许多与脂肪酸合成/三酰甘油（TAG）合成相关的基因（ACC、GPAT和DGAT）的mRNA水平升高。总之，类DpWRI1蛋白可将碳流转化为脂肪酸和三酰甘油（TAG）的生物合成。
Zotero 插件