Gamwell Jonathan M, Cresswell Emily, Pinnick Katherine E
Oxford Centre for Diabetes, Endocrinology and Metabolism, Radcliffe Department of Medicine, University of Oxford, Oxford, UK.
Methods Mol Biol. 2025;2938:31-46. doi: 10.1007/978-1-0716-4607-6_4.
In vitro models of adipogenesis are valuable tools for the study of human adipocyte development and function. Prevailing methods rely on the extended use of supraphysiological concentrations of glucose and adipogenic hormones, which can negatively impact some aspects of adipocyte function, in particular, insulin signaling. In this chapter, we present a modified adipogenic differentiation protocol which has been optimized for the study of insulin signaling and glucose uptake in human adipose-derived stem cells (hADSCs).
脂肪生成的体外模型是研究人类脂肪细胞发育和功能的宝贵工具。现有的方法依赖于超生理浓度的葡萄糖和脂肪生成激素的长期使用，这可能会对脂肪细胞功能的某些方面产生负面影响，特别是胰岛素信号传导。在本章中，我们提出了一种改良的脂肪生成分化方案，该方案已针对研究人类脂肪来源干细胞（hADSCs）中的胰岛素信号传导和葡萄糖摄取进行了优化。
