Peta Kimberly Thando, Pepper Michael S, Ambele Melvin A
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:119-123. doi: 10.1007/978-1-0716-4607-6_14.
The ability to isolate adipose-derived stromal/stem cells (ASCs) with relative ease has generated a great deal of interest in their potential use for regenerative medicine purposes. ASCs have shown promise in areas such as graft-versus-host disease and wound healing. Murine ASCs (mASCs) can be isolated from various adipose tissue sites. To further explore their therapeutic potential, it is crucial to be able to successfully isolate, expand, and characterize these cells. White adipose tissue (WAT) and brown adipose tissue are the main adipose tissue categories. This chapter presents a protocol for the isolation, expansion, and characterization of mASCs from excised WAT.
相对轻松地分离脂肪来源的基质/干细胞（ASC）的能力引发了人们对其在再生医学领域潜在用途的极大兴趣。ASC在移植物抗宿主病和伤口愈合等领域已显示出前景。小鼠ASC（mASC）可从各种脂肪组织部位分离得到。为了进一步探索其治疗潜力，成功分离、扩增和鉴定这些细胞至关重要。白色脂肪组织（WAT）和棕色脂肪组织是主要的脂肪组织类别。本章介绍了一种从切除的WAT中分离、扩增和鉴定mASC的方案。
