人脂肪来源干细胞（ASCs）的体外扩增

In Vitro Expansion of Human Adipose-Derived Stem Cells (ASCs).

作者信息

Adam Ladislaus Tapiwa, Ambele Melvin A, Pepper Michael S

机构信息

Institute for Cellular and Molecular Medicine, Department of Immunology and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.

出版信息

Methods Mol Biol. 2025;2938:103-108. doi: 10.1007/978-1-0716-4607-6_11.

DOI:10.1007/978-1-0716-4607-6_11

PMID:40445284

Abstract

Adipose-derived stromal/stem cells (ASCs) have raised a great deal of interest in research and regenerative medicine with potential applications in the treatment of several diseases. ASCs are often required in large numbers for use in research and clinical applications. Standardization of isolation methods, expansion, and characterization will ensure quality of the cells as well as safety in their use. This protocol describes the expansion and characterization of ASCs and highlights ASC viability assessment following expansion.

摘要

脂肪来源的基质/干细胞（ASC）在研究和再生医学领域引起了广泛关注，具有治疗多种疾病的潜在应用价值。在研究和临床应用中，通常需要大量的ASC。分离方法、扩增和鉴定的标准化将确保细胞质量及其使用的安全性。本方案描述了ASC的扩增和鉴定，并着重介绍了扩增后ASC活力评估。