Li Huilin, Ren Keying, He Chunlei, Hu Xiaochun, Sun Jingming, Wang Shuo, Pan Mingfei
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Key Laboratory of Food Quality and Health of Tianjin, Tianjin University of Science & Technology, 300457 Tianjin, China.
State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Key Laboratory of Food Quality and Health of Tianjin, Tianjin University of Science & Technology, 300457 Tianjin, China.
Food Chem. 2025 Oct 15;489:144906. doi: 10.1016/j.foodchem.2025.144906. Epub 2025 May 28.
Trace levels of pesticides and heavy metals in the environment and foodstuffs represent a substantial threat to human health. This study presented a bifunctional NH-MIL-88(Fe)@Pt nanozyme possessing superior peroxidase-mimicking activity and fluorescence properties, further establishing a dual-channel "OFF-ON" sensing platform for the simultaneous visual detection of glyphosate (GLY) and Pb. In the "OFF-ON" mechanism, cysteamine (CA)-mediated catalytic suppression of TMB oxidation was reversed through specific Pb chelation, enabling quantitative Pb detection with a limit of detection (LOD) of 0.0049 μg/mL (0.024 μM). The abundant -NH groups on NH-MIL-88(Fe)@Pt induced fluorescence quenching via Cu-triggered Lewis acid-base coordination. The preferential binding of Cu to GLY restored fluorescence, facilitating quantitative detection of GLY (LOD: 0.0082 μg/mL (0.048 μM)). Based on these principles, a hydrogel-based NH-MIL-88(Fe)@Pt nanozyme platform incorporating smartphone analysis was developed to achieve semi-quantitative visual detection of GLY and Pb, demonstrating significant potential for efficient contaminant screening in agri-environments and foodstuffs.
环境和食品中痕量的农药和重金属对人类健康构成重大威胁。本研究提出了一种具有优异过氧化物酶模拟活性和荧光特性的双功能NH-MIL-88(Fe)@Pt纳米酶，进一步建立了一种双通道“关闭-开启”传感平台，用于同时可视化检测草甘膦（GLY）和铅（Pb）。在“关闭-开启”机制中，通过特异性的铅螯合作用，半胱胺（CA）介导的对TMB氧化的催化抑制作用得以逆转，从而实现了铅的定量检测，检测限（LOD）为0.0049 μg/mL（0.024 μM）。NH-MIL-88(Fe)@Pt上丰富的-NH基团通过铜触发的路易斯酸碱配位诱导荧光猝灭。铜与草甘膦的优先结合恢复了荧光，便于对草甘膦进行定量检测（LOD：0.0082 μg/mL（0.048 μM））。基于这些原理，开发了一种结合智能手机分析的基于水凝胶的NH-MIL-88(Fe)@Pt纳米酶平台，以实现对草甘膦和铅的半定量可视化检测，显示出在农业环境和食品中高效污染物筛查的巨大潜力。
