Perpétuo Inês P, Bourne Lucie E, Orriss Isabel R
Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.
Methods Mol Biol. 2019;1914:21-38. doi: 10.1007/978-1-4939-8997-3_2.
This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.
本章介绍了成骨细胞的分离、培养和染色。该检测方法的主要优点在于，它能够直接测量骨基质沉积和矿化情况，并且在特定分化阶段产生大量成骨细胞用于分子和组织学分析。本章的另一个重点将是来自不太常见动物物种的成骨细胞培养。
