Quan Yu, Zhou Xin, Belmonte-Lopes Ricardo, Li Na, Wahyuningsih Retno, Chowdhary Anuradha, Hawksworth David L, Stielow J Benjamin, Walsh Thomas J, Zhang Sean, Teixeira Marcus de Melo, Matute Daniel, de Hoog Sybren, Wu Dong
Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China Affiliated Hospital of Guangdong Medical University Zhanjiang China.
Radboudumc/CWZ Center of Expertise for Mycology, Nijmegen, Netherlands Radboudumc/CWZ Center of Expertise for Mycology Nijmegen Netherlands.
IMA Fungus. 2025 May 23;16:e145658. doi: 10.3897/imafungus.16.145658. eCollection 2025.
The phylogeny of the vertebrate pathogen and its varieties was analyzed on the basis of GenBank data, comparing preceding papers that distinguished lineages on the basis of a much smaller dataset, partly dating back two decades. The aim was to establish the predictive value of individual research papers on biodiversity, which eventually may lead to altered nomenclature with large clinical consequences. A total of 1985 sequences of ITS, ARF, OLE and H-anti were downloaded. ITS showed insufficient resolution, and the sequences of the H-anti gene were too short to provide reliable conclusions. Ten major lineages from the seven reports were selected for comparison. Compared to the currently available global data, several earlier studies applied somewhat skewed datasets, biased towards the Americas. Possible separation of Indian and Indonesian lineages were consequently overlooked. Previously distinguished lineages were again recognized, but because of low bootstrap values and extensive genetic exchange, several of these do not deserve species status. No recombination was observed with North American and . An African clade (var. duboisii) was individualized. Despite its position in close association with South American clades, histopathology and clinical course of this entity underlines that it has other evolutionary drivers. This might also hold true for the North African donkey disease caused by var. farciminosum, although strains analyzed thus far are indistinguishable from South American strains. On the basis of phylogenetic data, Indian and Indonesian clades are separate, but more clinical data are needed to establish their value as individual species.
基于GenBank数据，对脊椎动物病原体及其变种的系统发育进行了分析，并与之前基于小得多的数据集（部分可追溯到二十年前）区分谱系的论文进行了比较。目的是确定关于生物多样性的个别研究论文的预测价值，这最终可能导致命名法的改变并产生重大临床后果。总共下载了1985条ITS、ARF、OLE和H-anti序列。ITS显示分辨率不足，H-anti基因的序列太短，无法得出可靠结论。从七份报告中选择了十个主要谱系进行比较。与目前可用的全球数据相比，一些早期研究使用的数据集有些偏差，偏向于美洲。因此，印度和印度尼西亚谱系的可能分离被忽视了。之前区分的谱系再次得到确认，但由于自展值较低和广泛的基因交换，其中一些谱系不值得作为物种地位。未观察到与北美和……的重组。一个非洲分支（杜氏变种）被区分出来。尽管它与南美分支密切相关，但其组织病理学和临床病程表明它有其他进化驱动因素。对于由伪皮疽变种引起的北非驴病可能也是如此，尽管迄今为止分析的菌株与南美菌株无法区分。根据系统发育数据，印度和印度尼西亚分支是分开的，但需要更多临床数据来确定它们作为独立物种的价值。
