Ishikawa Kemi, Hatai Hitoshi, Horie Masayuki, Ozawa Makoto, Tomioka Yukiko, Ijiri Moe, Tokorozaki Kaori, Ikeda Tomohito, Maruyama Satoshi, Esaki Mana, Fujimoto Yoshikazu
Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Farm Animal Clinical Skills and Disease Control Center, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan.
Transbound Emerg Dis. 2025 May 24;2025:2658800. doi: 10.1155/tbed/2658800. eCollection 2025.
Inclusion body disease of cranes (IBDCs) is fatal in many cases and reportedly caused by a herpes-like virus labeled as gruid herpesvirus 1 (GrHV-1). Although GrHV-1 has been isolated from IBDC-affected cranes, it has not been genetically classified because its genome has not been partially or fully sequenced. In this study, we isolated an alphaherpesvirus from hooded cranes () diagnosed with IBDC in Japan. Next-generation sequencing revealed that this virus isolate was GrHV-1, based on the 99.98% sequence homology with a previously isolated GrHV-1 strain. Furthermore, phylogenetic analysis of eight conserved herpesvirus genes supported the taxonomic assignment of GrHV-1 to the genus of the Alphaherpesvirinae subfamily. Based on these results, GrHV-1 can be more accurately classified and diagnostic tools to investigate suspected cases of IBDC can be developed. Furthermore, GrHV-1 showed effective replication in primary cultured cells derived from duck and chicken embryos and embryo tissues, highlighting the importance of further studies to evaluate its interspecies transmission.
鹤类包涵体病（IBDCs）在许多情况下是致命的，据报道是由一种被标记为鹤疱疹病毒1型（GrHV-1）的类疱疹病毒引起的。尽管GrHV-1已从感染IBDC的鹤中分离出来，但由于其基因组尚未进行部分或全序列测序，因此尚未进行基因分类。在本研究中，我们从日本诊断患有IBDC的白头鹤（）中分离出一种α疱疹病毒。下一代测序显示，该病毒分离株是GrHV-1，基于与先前分离的GrHV-1菌株99.98%的序列同源性。此外，对八个保守疱疹病毒基因的系统发育分析支持将GrHV-1分类到α疱疹病毒亚科的属。基于这些结果，可以更准确地对GrHV-1进行分类，并开发用于调查IBDC疑似病例的诊断工具。此外，GrHV-1在源自鸭和鸡胚胎及胚胎组织的原代培养细胞中显示出有效的复制，突出了进一步研究以评估其种间传播的重要性。
