Pfaff Florian, Schulze Christoph, König Patricia, Franzke Kati, Bock Sabine, Hlinak Andreas, Kämmerling Jens, Ochs Andreas, Schüle André, Mettenleiter Thomas C, Höper Dirk, Beer Martin
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald - Insel Riems, Germany.
Berlin-Brandenburg State Laboratory, Frankfurt (Oder), Germany.
J Gen Virol. 2017 Jan;98(1):89-95. doi: 10.1099/jgv.0.000698. Epub 2017 Feb 22.
A novel avian alphaherpesvirus, preliminarily designated sphenicid alphaherpesvirus 1 (SpAHV-1), has been independently isolated from juvenile Humboldt and African penguins (Spheniscus humboldti and Spheniscus demersus) kept in German zoos suffering from diphtheroid oropharyngitis/laryngotracheitis and necrotizing enteritis (collectively designated as penguin-diphtheria-like disease). High-throughput sequencing was used to determine the complete genome sequences of the first two SpAHV-1 isolates. SpAHV-1 comprises a class D genome with a length of about 164 kbp, a G+C content of 45.6 mol% and encodes 86 predicted ORFs. Taxonomic association of SpAHV-1 to the genus Mardivirus was supported by gene content clustering and phylogenetic analysis of herpesvirus core genes. The presented results imply that SpAHV-1 could be the primary causative agent of penguin-diphtheria-like fatal diseases in banded penguins. These results may serve as a basis for the development of diagnostic tools in order to investigate similar cases of penguin diphtheria in wild and captive penguins.
一种新型禽α疱疹病毒，初步命名为楔翼总目α疱疹病毒1（SpAHV-1），已从德国动物园饲养的患有类白喉性口咽炎/喉气管炎和坏死性肠炎（统称为企鹅类白喉样疾病）的洪堡企鹅和非洲企鹅（洪氏环企鹅和斑嘴环企鹅）幼崽中独立分离出来。利用高通量测序确定了最初两株SpAHV-1分离株的完整基因组序列。SpAHV-1包含一个长度约为164 kbp、G+C含量为45.6 mol%的D类基因组，编码86个预测的开放阅读框。通过基因内容聚类和疱疹病毒核心基因的系统发育分析，支持了SpAHV-1与马尔迪病毒属的分类学关联。所呈现的结果表明，SpAHV-1可能是带纹企鹅中类似企鹅白喉致命疾病的主要病原体。这些结果可为开发诊断工具提供基础，以便调查野生和圈养企鹅中类似企鹅白喉的病例。
