Zheng Ke, Zhou Xiaonan, Xu Ningyao, Liu Demei, Wang Haiqing
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 810008 Xining, China.
University of Chinese Academy of Sciences, 101408 Beijing, China.
Mol Breed. 2025 May 29;45(6):54. doi: 10.1007/s11032-025-01571-3. eCollection 2025 Jun.
Taigu genic male-sterile wheat, containing the dominant gene () , shows completely male-sterility in different genetic background and under various environments. The development of Aibai wheat with tightly linked and (), offers possibility for identifying the male-sterile plants by investigation of reduced plant height. However, due to the extreme dwarfism of Aibai wheat caused by , it inhabits a shadowed microenvironment caused by its fertile sibling plants. This results in delayed maturation of sterile plant progeny. In order to develop a novel germplasm with new visible marker for sorting male sterile plants conferred by , a binary vector containing , , , and driven by the aleurone-specific promoter was constructed and introduced into the wheat Fielder using -mediated transformation in this study. After investigation of the fertility, plant height and seed fluorescence of positive transgenic wheat plants, a line exhibited semi-dwarf male sterility, which could be reliably identified by the aleurone-specifically expressed red fluorescence in seeds, serving as a genetically stable reporter. Therefore, this study provides a novel male-sterile wheat that offers a powerful tool for hybrid seed production and facilitates genetic improvement in wheat through recurrent selection.
The online version contains supplementary material available at 10.1007/s11032-025-01571-3.
太谷核雄性不育小麦含有显性基因（），在不同遗传背景和各种环境下表现出完全雄性不育。紧密连锁的矮秆基因（）的矮败小麦的育成，为通过调查株高降低来鉴定雄性不育植株提供了可能。然而，由于矮秆基因导致矮败小麦极度矮化，其处于可育同胞植株造成的荫蔽微环境中，这导致不育植株后代成熟延迟。为了开发一种具有新可见标记的新型种质，用于筛选由矮秆基因赋予的雄性不育植株，本研究构建了一个由糊粉层特异性启动子驱动的含有、、和的二元载体，并通过介导转化将其导入小麦品种Fielder。在对阳性转基因小麦植株的育性、株高和种子荧光进行调查后，一个品系表现出半矮秆雄性不育，可通过种子中糊粉层特异性表达的红色荧光可靠地鉴定，作为一种遗传稳定的报告基因。因此，本研究提供了一种新型雄性不育小麦，为杂交种子生产提供了有力工具，并通过轮回选择促进了小麦的遗传改良。
在线版本包含可在10.1007/s11032-025-01571-3获取的补充材料。
