Altayr Nouralhuday Ali, Bukha Khawla Khirallah, Eteer Shahrazad Ali, El-Magrahi Najat Ali, Elhom Areeg M, Elmaloul Rayan A, Fakar Salma F
Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya.
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Open Vet J. 2025 Apr;15(4):1607-1614. doi: 10.5455/OVJ.2025.v15.i4.12. Epub 2025 Apr 30.
The bacterial load in fresh and frozen fish is a major public health concern.
This study aimed to determine the prevalence of human pathogenic foodborne bacteria and their antibiotic resistance profiles in fresh and frozen chub mackerel ( fish.
A total of 60 samples of fresh and imported frozen chub mackerel ( were randomly collected from anglers on boats in the Bab-Albahr market in Tripoli, Libya. Bacterial isolation from muscles was performed using a suitable culture media. Biochemical identification of the isolated bacteria was performed using the API 20E and its antibiogram.
The total number of bacterial isolates from 30 fresh samples was 53, and from 30 imported frozen samples was 26. The most frequently occurring species in fresh fish were (32%), (26.4%), (22.6%), and (18.8%), while in frozen fish were (50%), (23.07%), (15.3%), and (11.5%). The isolates exhibited high resistance to β-lactam antibiotics.
The microbial assessment of chub mackerel ( provides insights into the sanitary conditions along the Tripoli coast. In addition, the results of the present study indicate that imported frozen fish sold in Tripoli's fish markets could pose a public health threat. This research is considered a preliminary microbial assessment of contamination in the Tripoli market and along the Tripoli Coast. The incidence of S. aureus infections emphasized the presence of an issue in husbandry practices, insufficient adherence to basic hygiene protocols, and the random use of antibiotics.
新鲜和冷冻鱼类中的细菌载量是一个主要的公共卫生问题。
本研究旨在确定新鲜和冷冻鲐鱼中食源性致病菌的流行情况及其抗生素耐药谱。
从利比亚的黎波里Bab-Albahr市场的船上垂钓者处随机采集了60份新鲜和进口冷冻鲐鱼样本。使用合适的培养基对肌肉进行细菌分离。使用API 20E及其抗菌谱对分离出的细菌进行生化鉴定。
30份新鲜样本中分离出的细菌总数为53株，30份进口冷冻样本中为26株。新鲜鱼中最常见的菌种为（32%）、（26.4%）、（22.6%）和（18.8%），而冷冻鱼中为（50%）、（23.07%）、（15.3%）和（11.5%）。分离株对β-内酰胺类抗生素表现出高度耐药性。
鲐鱼的微生物评估为的黎波里海岸的卫生状况提供了见解。此外，本研究结果表明，在的黎波里鱼市场销售的进口冷冻鱼可能对公共卫生构成威胁。本研究被认为是对的黎波里市场和的黎波里海岸污染的初步微生物评估。金黄色葡萄球菌感染的发生率强调了养殖实践中存在的问题、对基本卫生规程的遵守不足以及抗生素的随意使用。
