Shin Eunkyung, Lee Dong-Yeop, Kim Min Kyeong, Yoo Jaeil, Hyeon Ji-Yeon, Kim Junyoung
Division of Bacterial Diseases, Bureau of Infectious Disease Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea.
Department of Veterinary Medicine, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
JAC Antimicrob Resist. 2025 May 30;7(3):dlaf090. doi: 10.1093/jacamr/dlaf090. eCollection 2025 Jun.
We investigated the epidemiological patterns of carbapenem-resistant, harbouring () ST 307 isolates in a tertiary hospital in South Korea between 2019 and 2021 and compared them to isolates collected from 25 hospitals through a nationwide survey, using whole genome sequencing and phylogenetic analysis.
Whole genome sequences of 25 isolates from hospital GB2 and 45 isolates from 25 other hospitals were analysed for antibiotic resistance gene profiles, virulence gene profiles, plasmid replicons, and phylogenetic relationships.
Core genome single nucleotide polymorphism (cgSNP)-based phylogenetic analysis categorized the 70 isolates into three distinct groups and 13 subgroups, supported by high bootstrap values and low SNP differences. Subgroup 1 comprised the 18 isolates from GB2 collected over 3 years, suggesting long-term persistence and repeated outbreaks within GB2. Region-specific dissemination was indicated by several subgroups containing isolates from different hospitals within the same province. Subgroup 7 comprised isolates from multiple provinces and years, suggesting potential long-distance transmission via patient transfers or community reservoirs. All isolates carried multiple β-lactam resistance genes; was observed in 67 isolates (89.3%) and the OXA variants in 70 isolates (93.3%). None of the isolates carried the or genes, while 58 of the 70 (82.9%) harboured yersiniabactin-encoding genes.
Our findings reveal both clonal persistence within a tertiary hospital and region-specific dissemination of producing ST307 across South Korea. These results highlight the importance of continuous nationwide surveillance and genomic analysis to implement effective infection control measures to mitigate further dissemination.
我们调查了2019年至2021年期间韩国一家三级医院中携带（）ST307的耐碳青霉烯类分离株的流行病学模式，并通过全基因组测序和系统发育分析将其与通过全国性调查从25家医院收集的分离株进行比较。
分析了来自GB2医院的25株分离株和来自其他25家医院的45株分离株的全基因组序列，以了解抗生素抗性基因谱、毒力基因谱、质粒复制子和系统发育关系。
基于核心基因组单核苷酸多态性（cgSNP）的系统发育分析将70株分离株分为三个不同的组和13个亚组，高自展值和低SNP差异支持了这一分类。亚组1包括在3年期间从GB2收集的18株分离株，表明GB2内存在长期持续性和反复爆发。几个亚组包含来自同一省内不同医院的分离株，表明存在区域特异性传播。亚组7包括来自多个省份和年份的分离株，表明可能通过患者转移或社区储存库进行远距离传播。所有分离株都携带多个β-内酰胺抗性基因；67株（89.3%）检测到，70株（93.3%）检测到OXA变体。没有分离株携带或基因，而70株中的58株（82.9%）携带编码yersiniabactin的基因。
我们的研究结果揭示了一家三级医院内的克隆持续性以及产ST307的菌株在韩国的区域特异性传播。这些结果凸显了持续进行全国性监测和基因组分析以实施有效感染控制措施以减轻进一步传播的重要性。
