Long Yixiu, Pei Xuan, Liu Hongyu, Ouyang Xueyan, Jiang Wei, Yang Huijuan
Department of Gynecological Oncology, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, 200032, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Hum Cell. 2025 Jun 2;38(4):115. doi: 10.1007/s13577-025-01225-8.
The pathogenesis of uterine carcinosarcoma (UCS) remains unclear due to a few mature cell lines. Herein, we established a new cell line, ESCA, from a Chinese woman. Especially, three sublines, named ESCA-2, ESCA-3, ESCA-5, were isolated based on the rate of cells' different sedimentation. All ESCA cells have been subcultured for more than 60 generations. ESCA sublines display different cell morphology and growth characteristics, as well as have different sensitivity to chemotherapeutic drugs. ESCA was most sensitive to paclitaxel and carboplatin, while ESCA-2 was most sensitive to ifosfamide. Besides, ESCA showed severe chromosome karyotype abnormalities and abnormal number of chromosomes. Whole exome sequence showed ESCA and its sublines, as well as tissue block shared similar single nucleotide variants, such as TP53, TRRAP mutations, while relatively large differences in mutational signature abundance. When all ESCA cells were xenotransplanted subcutaneously into BALB/c-nu mice, they developed into tumors that resembled the original tumor with positive AE1/3 and Vimentin in immunohistochemical staining. Interestingly, the transplanted tumor from ESCA-5 proliferated fastest with a relatively low level of glucose uptake evaluated by micro-PET/CT scanning. Taken together, ESCA and its sublines may be valuable tools to explore the molecular mechanism of UCS.
由于子宫癌肉瘤（UCS）的成熟细胞系较少，其发病机制仍不清楚。在此，我们从一名中国女性身上建立了一个新的细胞系ESCA。特别是，根据细胞不同沉降速率分离出了三个亚系，分别命名为ESCA - 2、ESCA - 3、ESCA - 5。所有ESCA细胞均已传代培养60代以上。ESCA亚系表现出不同的细胞形态和生长特性，对化疗药物的敏感性也不同。ESCA对紫杉醇和顺铂最敏感，而ESCA - 2对异环磷酰胺最敏感。此外，ESCA显示出严重的染色体核型异常和染色体数目异常。全外显子测序显示，ESCA及其亚系以及组织块具有相似的单核苷酸变异，如TP53、TRRAP突变，而突变特征丰度存在较大差异。当将所有ESCA细胞皮下异种移植到BALB/c - nu小鼠体内时，它们形成了与原发肿瘤相似的肿瘤，免疫组化染色显示AE1/3和波形蛋白呈阳性。有趣的是，通过微型PET/CT扫描评估，来自ESCA - 5的移植瘤增殖最快，葡萄糖摄取水平相对较低。综上所述，ESCA及其亚系可能是探索UCS分子机制的有价值工具。
