Seidel Zumra Peksaglam, Kumar Rridhisha, Conroy Araujo Mariana Mathias, Glass Sage A, Rodriguez Ronald, Goshia Tyler, Glass John I
J. Craig Venter Institute, San Diego, California 92037, United States.
Department of Cellular Biology, Institute of Biological Science, University of Brasília, Asa Norte, Brasília, Federal District 70910-900, Brazil.
ACS Synth Biol. 2025 Jun 20;14(6):1905-1911. doi: 10.1021/acssynbio.5c00159. Epub 2025 Jun 2.
The annual Minimal Cell Workshop, hosted by the J. Craig Venter Institute (JCVI), is an international virtual seminar that brings together over 80 academic, industrial, and government laboratories. Researchers use the JCVI's minimal bacterial cell platform to explore the principles of cellular life and integrate new chemical pathways. Since its creation in 2016, this platform has fostered global collaborations. The fourth workshop featured 26 talks on ongoing research with minimal cell strains, including JCVI-syn1.0, JCVI-syn3.0, JCVI-syn3A, and JCVI-syn3B. Topics included innovative imaging techniques like super-resolution microscopy and cryotomography, DNA replication assays, and minimal cell division. New approaches for ATP synthesis and the role of moonlighting proteins were also discussed. JCVI-syn3B applications explored its potential in understanding persistent pathogens and as an anticancer therapeutic. The workshop encouraged sharing techniques for cell culture and genetic manipulation, fostering collaboration and advancing efforts to develop genome-scale algorithms for understanding and manipulating cellular functions.
由J. 克雷格·文特尔研究所（JCVI）主办的年度最小细胞研讨会是一个国际虚拟研讨会，汇聚了80多个学术、工业和政府实验室。研究人员利用JCVI的最小细菌细胞平台来探索细胞生命的原理，并整合新的化学途径。自2016年创建以来，该平台促进了全球合作。第四届研讨会有26场关于最小细胞菌株（包括JCVI-syn1.0、JCVI-syn3.0、JCVI-syn3A和JCVI-syn3B）正在进行的研究的报告。主题包括超分辨率显微镜和冷冻断层扫描等创新成像技术、DNA复制测定以及最小细胞分裂。还讨论了ATP合成的新方法和兼职蛋白的作用。JCVI-syn3B的应用探索了其在理解持续性病原体方面的潜力以及作为抗癌疗法的潜力。该研讨会鼓励分享细胞培养和基因操作技术，促进合作，并推动开发用于理解和操纵细胞功能的基因组规模算法的努力。
