Ang Hui Ying, Subramani Tamilselvan, Yeap Swee Keong, Omar Abdul Rahman, Ho Wan Yang, Abdullah Mohd Puad, Alitheen Noorjahan Banu
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.
Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Exp Ther Med. 2014 Jun;7(6):1733-1737. doi: 10.3892/etm.2014.1657. Epub 2014 Apr 2.
Immunomodulators are agents that are able to stimulate or inhibit the immune response. The leaf extracts from and were analyzed for immunomodulatory activity and an MTT colorimetric assay was conducted to determine the proliferation of mice splenocytes and thymocytes. A bromodeoxyuridine assay was performed to analyze DNA synthesis and the Trypan blue exclusion method was conducted to evaluate the changes in total cell population. The results indicated that treatment with and produced a time- and dose-dependent increase in cell viability and proliferation. Following 72 h of treatment with and , thymocyte proliferation was augmented by 18 and 41%, respectively and splenocyte proliferation increased by 35 and 42%, respectively, when compared with untreated cells. The present study demonstrated that these extracts may act as potential immunostimulants and, thus, represent an alternative source of immunomodulatory compounds for the treatment of human immune-mediated diseases.
免疫调节剂是能够刺激或抑制免疫反应的物质。对[植物名称1]和[植物名称2]的叶提取物进行免疫调节活性分析，并进行MTT比色法以确定小鼠脾细胞和胸腺细胞的增殖情况。进行溴脱氧尿苷测定以分析DNA合成，并采用台盼蓝排斥法评估总细胞数量的变化。结果表明，用[植物名称1]和[植物名称2]处理可使细胞活力和增殖呈时间和剂量依赖性增加。与未处理的细胞相比，用[植物名称1]和[植物名称2]处理72小时后，胸腺细胞增殖分别增加了18%和41%，脾细胞增殖分别增加了35%和42%。本研究表明，这些提取物可能作为潜在的免疫刺激剂，因此，代表了用于治疗人类免疫介导疾病的免疫调节化合物的替代来源。
