Brzeziński Marek, Chmiela Magdalena, Picchio Matias, Calderón Marcelo, Gonciarz Weronika
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, Lodz 90-363, Poland.
Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, Lodz 90-237, Poland.
Langmuir. 2025 Jun 17;41(23):14915-14924. doi: 10.1021/acs.langmuir.5c01094. Epub 2025 Jun 3.
Terpene-based therapeutic eutectic solvents (THEES) represent an innovative class of green solvents with intrinsic antimicrobial properties, offering new potential for sustainable solutions in microbial infection treatment. This study introduces the first application of THEES-based microdroplets targeting (), a critical global health concern due to rising antibiotic resistance. Using microfluidic technology, we developed oil-in-water (O/W) emulsion droplets from three distinct THEES systems: menthol/thymol, menthol/lidocaine, and menthol/eucalyptol. These droplets were generated via a flow-focusing glass-capillary microfluidic device, with a 10 wt % poly(vinyl alcohol) (PVA) aqueous phase and THEES as the organic phase. The presence of surfactant in the system is expected to diminish the integration of bacterial membranes and improve the antimicrobial efficiency of the emulsion droplets compared to THEES alone. Biocompatibility was first evaluated metabolic activity on L929 mouse fibroblasts, followed by testing evaluation against both standard and antibiotic-resistant strains. This novel platform THEES-based microdroplets offers a sustainable and potentially transformative strategy for addressing infections, providing a breakthrough in combating antibiotic resistance and can potentially prevent the development of resistance if it is used as an alternative to antibiotics.
基于萜烯的治疗性低共熔溶剂（THEES）是一类具有内在抗菌特性的新型绿色溶剂，为微生物感染治疗的可持续解决方案提供了新的潜力。本研究首次介绍了基于THEES的微滴在治疗（）方面的应用，（）由于抗生素耐药性上升已成为全球关键的健康问题。利用微流控技术，我们从三种不同的THEES系统（薄荷醇/百里酚、薄荷醇/利多卡因和薄荷醇/桉叶油素）中制备了水包油（O/W）乳液微滴。这些微滴通过流动聚焦玻璃毛细管微流控装置产生，以10 wt%的聚乙烯醇（PVA）水相和THEES作为有机相。与单独的THEES相比，系统中表面活性剂的存在预计会减少细菌膜的整合并提高乳液微滴的抗菌效率。首先在L929小鼠成纤维细胞上通过代谢活性评估生物相容性，随后针对标准菌株和抗生素耐药菌株进行测试评估。这种基于THEES微滴的新型平台为解决（）感染提供了一种可持续且可能具有变革性的策略，在对抗抗生素耐药性方面取得了突破，如果用作抗生素的替代品，有可能防止耐药性的产生。
