Jimmy Jini, Puthumana Jayesh, Joseph Valsamma, Singh I S Bright
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin, Kerala, 682016, India.
Curr Microbiol. 2025 Jun 3;82(7):322. doi: 10.1007/s00284-025-04279-x.
Pyocyanin, the nitrogen-containing heterocyclic phenazine compound produced by Pseudomonas aeruginosa, has been proven to be a potential drug of choice against vibriosis in sustainable aquaculture production systems. Considering the cost of production and the fact that pyocyanin is produced in very small quantities in nature genetic improvement of bacterial strains for enhanced production of pyocyanin is a requirement. In-depth studies on the key biomolecules behind the biosynthesis of pyocyanin in P.aeruginosa has been conducted previously, however, gene level manipulations of the pyocyanin biosynthetic pathway has to be experimented enhancing the yield in P.aeruginosa. In this review, we look in to the molecular mechanism behind pyocyanin production in P. aeruginosa and an attempt has been made to investigate the growing scope of microbial synthetic biology in pyocyanin biosynthesis. In the present scenario where, synthetic biology-based tools are gaining much importance, this review throws light to building up a platform for synthetic biology-based production of pyocyanin.
绿脓菌素是由铜绿假单胞菌产生的含氮杂环吩嗪化合物，已被证明是可持续水产养殖生产系统中对抗弧菌病的一种潜在首选药物。考虑到生产成本以及绿脓菌素在自然界中产量极低这一事实，对细菌菌株进行基因改良以提高绿脓菌素产量是必要的。此前已对铜绿假单胞菌中绿脓菌素生物合成背后的关键生物分子进行了深入研究，然而，必须对绿脓菌素生物合成途径进行基因水平的操作，以提高铜绿假单胞菌中的产量。在这篇综述中，我们探究了铜绿假单胞菌中绿脓菌素产生的分子机制，并尝试研究微生物合成生物学在绿脓菌素生物合成中不断扩大的应用范围。在基于合成生物学的工具日益重要的当前情况下，这篇综述为建立基于合成生物学生产绿脓菌素的平台提供了思路。
