Bochko Tatiana, Andrusenko Elena, Glushakov Ruslan, Melnikova Galina, Shishov Andrey
Department of Analytical Chemistry, Institute of Chemistry, Saint-Petersburg University, Saint Petersburg State University, SPbSU, SPbU, 7/9 Universitetskaya nab., St. Petersburg, 199034, Russia.
Military Medical Academy n.a. S.M. Kirov of Ministry of defense of the Russian Federation, Akademika Lebedeva st., 6, St. Petersburg, 194044, Russia.
Anal Bioanal Chem. 2025 Jun 4. doi: 10.1007/s00216-025-05941-w.
Rubia tinctorum L. roots are a valuable source of anthraquinones and other bioactive phytochemicals. In this study, nine hydrophilic deep eutectic solvents (DESs), based on choline chloride or tetrabutylammonium bromide, were evaluated as green extractants for the selective recovery of bioactive compounds. Their performance was compared with four conventional solvents (acetonitrile, methanol, ethanol-water, and water). Among DESs, choline chloride-lactic acid and choline chloride-malonic acid showed the highest extraction efficiency for gallic acid, caffeine, quercetin, alizarin, and purpurin. Extraction conditions were optimized using response surface methodology. The antioxidant, antimicrobial, and phytochemical profiles of the resulting extracts were comprehensively evaluated. GC-MS analysis revealed the presence of key metabolites including alizarin, rubiadin, purpurin, scillarenin, and fucosterol. The results demonstrate the potential of hydrophilic DESs as sustainable, efficient, and selective media for the extraction of biologically active compounds from plant materials.
茜草的根是蒽醌类化合物和其他具有生物活性的植物化学物质的宝贵来源。在本研究中，评估了九种基于氯化胆碱或溴化四丁基铵的亲水性深共熔溶剂（DESs）作为绿色萃取剂用于选择性回收生物活性化合物。将它们的性能与四种传统溶剂（乙腈、甲醇、乙醇 - 水和水）进行了比较。在DESs中，氯化胆碱 - 乳酸和氯化胆碱 - 丙二酸对没食子酸、咖啡因、槲皮素、茜素和红紫素表现出最高的萃取效率。使用响应面法对萃取条件进行了优化。对所得提取物的抗氧化、抗菌和植物化学特征进行了全面评估。气相色谱 - 质谱分析揭示了关键代谢物的存在，包括茜素、茜草素、红紫素、海葱苷元和岩藻甾醇。结果表明亲水性DESs作为从植物材料中提取生物活性化合物的可持续、高效和选择性介质的潜力。
