天然深共晶溶剂在提取（L.）生物活性成分中的应用。

Application of Natural Deep Eutectic Solvents for Extraction of Bioactive Components from (L.).

机构信息

Tananaev Institute of Chemistry and Technology of Rare Elements and Mineral Raw Materials-Subdivision of the Federal Research Centre «Kola Science Centre of the Russian Academy of Sciences», Akademgorodok 26a, 184209 Apatity, Russia.

Chemistry Department, Murmansk State Technical University, Sportivnaja Str. 13, 183010 Murmansk, Russia.

出版信息

Molecules. 2023 Jan 16;28(2):912. doi: 10.3390/molecules28020912.

(L.) is a valuable source of nutrients. Nutrients have adaptogenic, immunostimulating, nootropic, anti-inflammatory and anti-cancer properties. Natural deep eutectic solvents (NADES) consisting of choline chloride and malonic, malic, tartaric or citric acids have been first used to extract biologically active substances from . The total content of polyphenols has been determined by the Folin-Ciocalteu method for all extracts. Antioxidant activity has been determined by the phosphomolybdate method, and antiradical activity has been determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rosavin concentration has been determined by high-performance liquid chromatography (HPLC). Extraction kinetics has been evaluated regarding the effectiveness of NADES with each other and with reference solvents (water and 50% ethanol) has been made. Extraction conditions have been optimized according to the Box-Behnken design of the experiment. The optimal parameters of the extraction process have been established. The antibacterial activity of NADES-based extracts against bacterial cultures of , , and has been studied.

（L.）是一种有价值的营养来源。营养素有适应原、免疫刺激、益智、抗炎和抗癌特性。由氯化胆碱和丙二酸、苹果酸、酒石酸或柠檬酸组成的天然深共晶溶剂（NADES）首次被用于从中提取具有生物活性的物质。所有提取物的多酚总含量均采用 Folin-Ciocalteu 法测定。抗氧化活性采用磷钼酸盐法测定，自由基清除活性采用 2,2-二苯基-1-苦肼基（DPPH）法测定。采用高效液相色谱法（HPLC）测定罗莎文浓度。评估了 NADES 之间以及与参考溶剂（水和 50%乙醇）的提取效率，并根据实验的 Box-Behnken 设计进行了优化。确定了提取工艺的最佳参数。研究了基于 NADES 的提取物对、、和的细菌培养物的抗菌活性。