Sodéré Paul, Somda Marius K, Zongo Luc, Mihin Henriette B, Mogmenga Iliassou, Akakpo Agbémébia Y, Dicko Mamoudou H
Laboratory of Microbiology and Microbial Biotechnology, Department of Biochemistry and Microbiology, University Joseph KI-ZERBO, Ouagadougou, 03 BP: 7021, Burkina Faso.
Laboratory of Biochemistry Biotechnology Food Technology and Nutrition, Department of Biochemistry Microbiology, University Joseph KI-ZERBO, Ouagadougou, 03 BP: 7021, Burkina Faso.
Infect Drug Resist. 2025 May 30;18:2743-2763. doi: 10.2147/IDR.S518717. eCollection 2025.
Bacterial resistance to antibiotics is an important developing threat for human health. Therefore, searching for alternatives through the synergistic combination of essential oils with conventional antibiotics is one of the relevant approaches. This study aims to investigate the mechanism of action of essential oils isolated from local common medicinal plants of Burkina Faso ( and ) combined with three antibiotics (ciprofloxacin, amoxicillin + clavulanic acid and colistin) against resistant-bacterial strains (08) involved in toxi-intoxic effects ( ATCC 2523, ATCC 25922, ATCC 9027, 0366 V, SKN 1152, 0998 V, 0486 V, 0938 V).
The methodological approach consisted of evaluating the antibacterial activity followed by investigating antibacterial mechanism.
The MICs (minimum inhibitory concentration) of and ranged from 4.05 mg/mL to 64.92 mg/mL and from 9.57 mg/mL to 38.28 mg/mL, respectively. Checkerboard assays revealed synergistic effects resulting in reductions of 93.69% and 87.73%, respectively, in amoxicillin+ clavulanic acid and colistin MICs. Protein and nucleic acid leakage assays demonstrated that peptidoglycan and cytoplasmic membrane damage induced by the synergistic combination were significantly greater than those in the control. The viable count of bacteria for this combination showed a complete killing profile after 12 hours and further.
It appears from this study that the combinations (essential oil-antibiotic) reduced the MICs of the antibiotics and eradicated antibiotic-resistant bacteria completely. This combination could constitute an excellent means against bacterial resistance to antibiotics.
细菌对抗生素的耐药性是人类健康面临的一个重要且不断发展的威胁。因此，通过将精油与传统抗生素协同组合来寻找替代方法是相关途径之一。本研究旨在探究从布基纳法索当地常见药用植物中分离出的精油（和）与三种抗生素（环丙沙星、阿莫西林+克拉维酸和黏菌素）联合作用于涉及毒性中毒效应的耐药菌株（08）（美国典型培养物保藏中心2523、美国典型培养物保藏中心25922、美国典型培养物保藏中心9027、0366V、SKN 1152、0998V、0486V、0938V）的作用机制。
该方法包括评估抗菌活性，随后研究抗菌机制。
和的最低抑菌浓度（MIC）分别为4.05毫克/毫升至64.92毫克/毫升以及9.57毫克/毫升至38.28毫克/毫升。棋盘法分析显示协同效应，分别使阿莫西林+克拉维酸和黏菌素的MIC降低了93.69%和87.73%。蛋白质和核酸泄漏分析表明，协同组合诱导的肽聚糖和细胞质膜损伤明显大于对照组。该组合对细菌的活菌计数显示12小时及之后呈现完全杀灭情况。
从本研究来看，（精油-抗生素）组合降低了抗生素的MIC并完全根除了耐药细菌。这种组合可能构成对抗细菌抗生素耐药性的一种极佳手段。
