Liu Ping, Honda Masayuki, Kawano Ryuji
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, 184-8588, Japan.
Department of Electrical Engineering and Computer Science, Tokyo University of Agriculture and Technology, Tokyo, 184-8588, Japan.
Small Methods. 2025 Aug;9(8):e2401760. doi: 10.1002/smtd.202401760. Epub 2025 Jun 4.
A method for detecting DNA methylation and modifications is developed using biological nanopores. By exploiting the interaction between bases and acidic amino acids within the entrance and neck region of the α-hemolysin nanopore, we determined the position and frequency of 5-methylcytosine in oligonucleotides. Furthermore, the detection of demethylation intermediates is optimized by examining various ion species and concentrations in the electrolyte. Efforts are also made to employ commercial nanopore devices for high-throughput detection. This approach offers the potential for direct detection of DNA methylation and modifications using biological nanopores.
一种利用生物纳米孔检测DNA甲基化和修饰的方法被开发出来。通过利用碱基与α-溶血素纳米孔入口和颈部区域内酸性氨基酸之间的相互作用，我们确定了寡核苷酸中5-甲基胞嘧啶的位置和频率。此外，通过检查电解质中的各种离子种类和浓度，优化了对去甲基化中间体的检测。还努力采用商业纳米孔设备进行高通量检测。这种方法为利用生物纳米孔直接检测DNA甲基化和修饰提供了可能性。
