Tian Ye, Wang Xiaojuan, Lu Qian
Hepatopancreatobiliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, 102218, Beijing, China.
Institute for Organ Transplant and Bionic Medicine, Beijing Key Laboratory of Liver Transplantation and Bionic Manufacturing, Tsinghua University, 102218, Beijing, China.
J Cancer Res Clin Oncol. 2025 Sep 13;151(9):257. doi: 10.1007/s00432-025-06299-3.
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Liver transplantation (LT) remains a vital treatment for HCC, yet it still faces numerous challenges in patient selection, recurrence monitoring, and personalized therapy. Third-generation sequencing (TGS), with its advantages of long read length, high throughput, direct detection of epigenetic modifications, real-time analysis and high accuracy, offers promise for advancing precision medicine in LT. While previous reviews have focused on TGS technical features, this review uniquely synthesizes its role in addressing specific clinical challenges in LT-HCC management and critically assesses its translational pathway. Specifically, it systematically examines TGS applications in candidate screening, recurrence monitoring, and personalized therapy for LT-HCC, analyzes its prospects for clinical translation, and aims to provide new insights for precise HCC treatment. We also discuss barriers to implementation and suggest solutions for integrating TGS into clinical workflows. This review provides a roadmap for leveraging TGS to revolutionize precision medicine in transplant hepatology.
肝细胞癌（HCC）是全球癌症相关死亡的主要原因之一。肝移植（LT）仍然是HCC的重要治疗方法，但在患者选择、复发监测和个性化治疗方面仍面临诸多挑战。第三代测序（TGS）具有读长较长、高通量、直接检测表观遗传修饰、实时分析和高精度等优点，为推进肝移植精准医学带来了希望。虽然以往的综述主要关注TGS的技术特征，但本综述独特地综合了其在解决LT-HCC管理中特定临床挑战方面的作用，并对其转化途径进行了批判性评估。具体而言，它系统地研究了TGS在LT-HCC候选者筛选、复发监测和个性化治疗中的应用，分析了其临床转化前景，旨在为精确的HCC治疗提供新见解。我们还讨论了实施障碍，并提出了将TGS整合到临床工作流程中的解决方案。本综述为利用TGS彻底改变移植肝病学中的精准医学提供了路线图。
