Laurent Julie M, Steinacher Mathias, Kan Anton, Ritter Maximilian, Leutert Mario, Bienz Siiri, Häberlin David, Kumar Naresh, Studart André R
Complex Materials, Department of Materials, ETH Zürich, Zürich, 8093, Switzerland.
Wood Materials Science, Institute for Building Materials, Department of Civil, Environmental and Geomatic Engineering, ETH Zürich, Zürich, 8093, Switzerland.
Adv Sci (Weinh). 2025 Sep;12(33):e05075. doi: 10.1002/advs.202505075. Epub 2025 Jun 5.
The synthesis of cellulose pellicles by bacteria offers an enticing strategy for the biofabrication of sustainable materials and biomedical devices. To leverage this potential, bacterial strains that overproduce cellulose are identified through directed evolution technology. While cellulose overproduction is linked with a specific genetic mutation, the effect of such mutation on the intracellular protein landscape and on the structure and mechanical properties of the cellulose pellicles is not yet understood. Here, the proteome of bacteria evolved to overproduce cellulose is studied and its effect on the structure and mechanics of the resulting cellulose pellicles is investigated. Proteomic analysis reveals that the protein landscape of the evolved bacteria shows pronounced differences from that of native microorganisms. Thanks to concerted changes in the proteome, the evolved bacteria can generate cellulose pellicles with exquisite structure and improved mechanical properties for applications in textiles, packaging, and medical implants.
细菌合成纤维素薄膜为可持续材料和生物医学设备的生物制造提供了一种诱人的策略。为了利用这一潜力，通过定向进化技术鉴定出过量生产纤维素的细菌菌株。虽然纤维素的过量生产与特定的基因突变有关，但这种突变对细胞内蛋白质格局以及纤维素薄膜的结构和力学性能的影响尚不清楚。在此，研究了进化出过量生产纤维素能力的细菌的蛋白质组，并研究了其对所得纤维素薄膜的结构和力学性能的影响。蛋白质组学分析表明，进化细菌的蛋白质格局与天然微生物有明显差异。由于蛋白质组的协同变化，进化细菌能够生成结构精致、力学性能改善的纤维素薄膜，可用于纺织品、包装和医疗植入物等领域。
