Yarzabal L A, Bout D, Naquira F, Fruit J, Andrieu S
Sabouraudia. 1977 Mar;15(1):79-85.
A new purified antigen (E2) of Paracoccidioides brasiliensis mycelial growth phase was isolated by immunoadsorption from a crude metabolic soluble extract of the fungus. The antiserum prepared in a rabbit by inoculation of E2 antigen developed only one immunodiffusion line with the crude metabolic extract. Findings on immunological analysis showed that E2 antigen is the antigenic component of immunoelectrophoretic band E. The isolated antigens did not possess detectable alkaline phosphatase activity. It reacted in immunodiffusion tests with all the sera (14/14) from P. brasiliensis infected patients containing precipitating antibodies.
通过免疫吸附从巴西副球孢子菌菌丝体生长阶段的粗代谢可溶性提取物中分离出一种新的纯化抗原（E2）。用E2抗原接种兔子制备的抗血清与粗代谢提取物仅产生一条免疫扩散线。免疫分析结果表明，E2抗原是免疫电泳带E的抗原成分。分离出的抗原不具有可检测到的碱性磷酸酶活性。它在免疫扩散试验中与所有来自感染巴西副球孢子菌且含有沉淀抗体的患者血清（14/14）发生反应。
