Rodrigues E G, Travassos L R
Disciplina de Biologia Celular, Escola Paulista de Medicina, São Paulo, Brazil.
J Med Vet Mycol. 1994;32(1):77-81.
Fava Nettos' polysaccharide antigen (FNPA), shown to detect humoral and cellular responses in paracoccidioidomycosis, was investigated. Skin tests with FNPA were negative after alkaline hydrolysis or depletion of gp43 peptide epitopes. Purified antibodies to FNPA or gp43 from paracoccidioidomycosis patients cross-reacted, showing common epitopes and FNPA-specific ones. Normal human sera, in contrast to paracoccidioidomycosis sera, were unreactive with gp43 but recognized epitopes of FNPA susceptible to alkaline hydrolysis and pronase treatment. Histoplasmosis patients sera strongly reacted with FNPA carbohydrate epitopes.
对已证明可检测副球孢子菌病中体液和细胞反应的法瓦内托斯多糖抗原（FNPA）进行了研究。用碱性水解或耗尽gp43肽表位后，FNPA皮肤试验呈阴性。来自副球孢子菌病患者的纯化抗FNPA或抗gp43抗体发生交叉反应，显示出共同表位和FNPA特异性表位。与副球孢子菌病血清相比，正常人血清与gp43无反应，但能识别易受碱性水解和链霉蛋白酶处理影响的FNPA表位。组织胞浆菌病患者血清与FNPA碳水化合物表位强烈反应。
