Çorak Mehmet, Kaya Mumcu Arzu, Gülbandılar Aysel
Department of Endodontics, Faculty of Dentistry, Kutahya Health Sciences University, Kutahya, Turkey.
Department of Food Engineering, Faculty of Agriculture, Eskisehir Osmangazi University, Eskisehır, Turkey.
Sci Rep. 2025 Jun 5;15(1):19862. doi: 10.1038/s41598-025-03072-x.
This study aimed to evaluate the antibacterial efficacy of calcium hydroxide (Ca(OH)), Ca(OH) + resveratrol (RV), Ca(OH) + silver nanoparticles(AgNPs), and Ca(OH) + salicylic acid (SA) against Enterococcus faecalis (E. faecalis) using culture methods and field emission scanning electron microscopy (FE-SEM). Fifty-four extracted single-rooted teeth were prepared with standardized internal resorption cavities and inoculated with E. faecalis (ATCC 29212). After 21 days of incubation, baseline bacterial counts were determined. The teeth were randomly assigned to five groups (n = 10) based on the applied medicament. After 14 days of incubation, bacterial counts were reassessed, and two samples from each group were analyzed using FE-SEM. Data were analyzed using one-way ANOVA and Duncan's test (p < .05). All medicament groups significantly reduced bacterial counts (p < .001), with Ca(OH) + RV, Ca(OH) + AgNPs, and Ca(OH) + SA demonstrating superior antibacterial activity compared to Ca(OH) alone (p < .001). The saline group exhibited the highest bacterial counts (p < .001). Combining Ca(OH)₂ with RV, AgNPs, or SA significantly enhances antibacterial efficacy in internal root resorption cases. These combinations may serve as effective alternatives to Ca(OH) alone in endodontic disinfection. Intracanal medicament application plays a vital role in eliminating microorganisms and enhancing the success of endodontic treatment, particularly in cases of internal root resorption. This study highlights the superior antibacterial efficacy of Ca(OH) combined with RV, AgNPs, or SA against E. faecalis. These findings suggest that incorporating these adjuncts into intracanal medicaments may improve disinfection outcomes, offering potential clinical benefits in managing internal root resorption cases.
本研究旨在通过培养方法和场发射扫描电子显微镜（FE-SEM）评估氢氧化钙（Ca(OH)）、Ca(OH) + 白藜芦醇（RV）、Ca(OH) + 银纳米颗粒（AgNPs）和Ca(OH) + 水杨酸（SA）对粪肠球菌（E. faecalis）的抗菌效果。制备了54颗单根离体牙，形成标准化的内吸收洞，并接种粪肠球菌（ATCC 29212）。孵育21天后，测定基线细菌计数。根据所应用的药物，将牙齿随机分为五组（n = 10）。孵育14天后，重新评估细菌计数，并使用FE-SEM对每组的两个样本进行分析。数据采用单因素方差分析和邓肯检验（p < 0.05）。所有药物组的细菌计数均显著降低（p < 0.001），与单独使用Ca(OH)相比，Ca(OH) + RV、Ca(OH) + AgNPs和Ca(OH) + SA表现出更强的抗菌活性（p < 0.001）。生理盐水组的细菌计数最高（p < 0.001）。在根管内吸收病例中，将Ca(OH)₂与RV、AgNPs或SA联合使用可显著提高抗菌效果。这些联合用药可能是单独使用Ca(OH)进行根管消毒的有效替代方法。根管内药物应用在消除微生物和提高根管治疗成功率方面起着至关重要的作用，尤其是在根管内吸收病例中。本研究强调了Ca(OH)与RV、AgNPs或SA联合使用对粪肠球菌具有更强的抗菌效果。这些发现表明，将这些辅助剂纳入根管内药物可能会改善消毒效果，为处理根管内吸收病例提供潜在的临床益处。
