Department of Conservative Dentistry and Endodontics, Saveetha Dental College & Hospitals, Saveetha University, Chennai, Tamilnadu, India.
Department of Conservative Dentistry and Endodontics, SRM Dental College, Ramapuram, Chennai, Tamilnadu, India.
BMC Oral Health. 2023 Nov 11;23(1):849. doi: 10.1186/s12903-023-03552-4.
The study aims to compare the synergistic antibacterial efficacy of different combinations of calcium hydroxide as an intracanal medicament against E. faecalis.
The current study included four hundred extracted human permanent mandibular premolar teeth. After complete chemo-mechanical preparation, the middle third of the root was sectioned using a rotary diamond disc and a total of 400 samples were obtained. The specimens were inoculated with E. faecalis for 21 days. After that, specimens were divided into five groups (n = 80) based on materials used for the disinfection of samples: Group I, calcium hydroxide alone; Group II, calcium hydroxide + 2% chlorhexidine gel; Group III, calcium hydroxide + 2% chitosan gel; Group IV, calcium hydroxide + 0.02% silver nanoparticle gel; Group V, calcium hydroxide + Bioactive glass S53P4. Dentin shavings from the apical third were obtained from the inner third of dentin were obtained using gates glidden no.1 to the apical depth, followed by no.2, 3, 4 and 5 analyzed for E. faecalis using the culture method. One-way analysis of variance (ANOVA) was used for data analysis, followed by post-hoc Tukey's test for multiple comparisons of means to check the difference in bacterial inhibition between the groups.
ANOVA results revealed a significant reduction of bacterial counts in all the groups compared (p < 0.001). Intergroup comparison showed maximum bacterial reduction (p < 0.001) with calcium hydroxide + bioactive glass S53P4 compared with other groups.
Synergistic effect of calcium hydroxide showed better bacterial reduction compared to calcium hydroxide alone. Among the combinations evaluated, calcium hydroxide with bioactive glass, found to be most effective compared to other groups.
本研究旨在比较氢氧化钙作为根管内药物对粪肠球菌的协同抗菌效果。
本研究共纳入 400 颗人下颌前磨牙。完成化学机械预备后，使用旋转金刚石片将根尖三分之一部分切开，共获得 400 个样本。将样本接种粪肠球菌 21 天。之后，根据用于样本消毒的材料将样本分为五组（n=80）：组 I：氢氧化钙单独使用；组 II：氢氧化钙+2%洗必泰凝胶；组 III：氢氧化钙+2%壳聚糖凝胶；组 IV：氢氧化钙+0.02%载银纳米凝胶；组 V：氢氧化钙+生物活性玻璃 S53P4。用 Gates Glidden 号 1 从内三分之一牙本质中获得根尖三分之一的牙本质屑，直至根尖深度，然后用 2、3、4、5 号进行分析，采用培养法检测粪肠球菌。采用单因素方差分析（ANOVA）进行数据分析，然后采用 Tukey 事后检验进行多组间均值比较，以检查各组间细菌抑制的差异。
ANOVA 结果显示，所有组的细菌计数均显著降低（p<0.001）。组间比较显示，与其他组相比，氢氧化钙+生物活性玻璃 S53P4 组的细菌减少最多（p<0.001）。
与单独使用氢氧化钙相比，氢氧化钙的协同作用显示出更好的细菌减少效果。在所评估的组合中，与其他组相比，发现氢氧化钙与生物活性玻璃的组合最有效。
