Guerra Janaína Motta, Albuquerque Maria Carolina Portela, de Araújo Clarice Lioba, Montenegro Júlia Gonçalves, Cunha Diana Araújo, Sousa Fabrício Bitu, Filho Paulo Goberlânio de Barros, Nobre Átila Vinícius Vitor
Department of Dentistry, Christus University Center, João Adolfo Gurgel Street, 133, Cocó, Fortaleza, CE, 60192-345, Brazil.
Aesthetic Plast Surg. 2025 Jun 5. doi: 10.1007/s00266-025-04970-1.
The objective of the present work was to evaluate the percentage of neocollagenesis induction of the Sculptra in different forms of reconstitution.
For this, 150 male Swiss mice were used to which saline solution or reconstituted Sculptra were applied to the back subcutaneous tissue immediately before application (0 h), 2, 24 or 72 h before administration and were euthanized after 15, 30 and 60 days of administration of the material in a number of 10/group/day. Their backs were removed for histological analysis and immunoexpression for alpha-smooth muscle actin (α-SMA). The data was statistically analyzed (p < 0.05).
After 30 days of administration, the Sculptra 72 h group showed greater collagen formation than the control (p = 0.016) and 0h groups (p = 0.023). After 60 days, the Sculptra 24 and 72 h groups also showed greater collagen formation than the control (p = 0.025) and 0 h groups ( p = 0.039). After 30 days of administration, the Sculptra 24 and 72 h groups showed higher immunoexpression for α-SMA (p = 0.001), as well as after 60 days (p < 0.001).
We can conclude that Sculptra reconstituted 2, 24 and 72 h before its application has the potential to induce collagen after 60 days. Furthermore, there is greater expression of α-SMA after reconstitution 24 and 72 h before Sculptra injection, after 30 and 60 days. No level of evidence is needed for Basic Science, Animal Study, Cadaver Study, and Experimental Study Articles.
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本研究的目的是评估不同复溶形式的塑然雅诱导新胶原生成的百分比。
为此，使用150只雄性瑞士小鼠，在给药前即刻（0小时）、给药前2小时、24小时或72小时将生理盐水或复溶的塑然雅应用于背部皮下组织，在给予材料15天、30天和60天后每组每天处死10只小鼠。取下它们的背部进行组织学分析和α-平滑肌肌动蛋白（α-SMA）的免疫表达分析。对数据进行统计学分析（p<0.05）。
给药30天后，塑然雅72小时组的胶原形成比对照组（p=0.016）和0小时组（p=0.023）更多。给药60天后，塑然雅24小时组和72小时组的胶原形成也比对照组（p=0.025）和0小时组（p=0.039）更多。给药30天后，塑然雅24小时组和72小时组的α-SMA免疫表达更高（p=0.001），给药60天后也是如此（p<0.001）。
我们可以得出结论，在应用前2小时、24小时和72小时复溶的塑然雅在60天后有诱导胶原生成的潜力。此外，在塑然雅注射前24小时和72小时复溶后30天和60天，α-SMA的表达更高。基础科学、动物研究、尸体研究和实验研究文章不需要证据水平。
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