Shen Yusheng, Yan Caishan, Huang Pingbo, Ori-McKenney Kassandra M, Lai Pik-Yin, Tong Penger
Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA 95616, USA.
bioRxiv. 2025 May 23:2025.05.19.654995. doi: 10.1101/2025.05.19.654995.
Cargo transport within cells is a vital biological process that relies on the intricate interplay between motor proteins, microtubules, and the complex intracellular environment. In this study, we unveil a universal transport mechanism characterized by stick-slip motion, which governs the dynamics of intracellular vesicle transport. By analyzing a comprehensive dataset of vesicle trajectories across various cell types and intracellular environments, we demonstrate that the cargo velocities consistently follow a Gamma distribution, revealing a common statistical pattern amidst the diversity of biological cargoes. Our experimental findings are well-described by a theoretical model that connects the Brownian-correlated kinetic friction between motor-cargo complexes and their surroundings to the observed universal Gamma distribution of cargo velocities. This model elucidates the stick-slip dynamics governing intracellular cargo transport, which are pertinent to various cellular processes such as vesicle budding, organelle transport, and cell migration.
细胞内的货物运输是一个至关重要的生物学过程，它依赖于驱动蛋白、微管和复杂的细胞内环境之间的复杂相互作用。在本研究中，我们揭示了一种以粘滑运动为特征的通用运输机制，该机制控制着细胞内囊泡运输的动力学。通过分析跨各种细胞类型和细胞内环境的囊泡轨迹的综合数据集，我们证明货物速度始终遵循伽马分布，揭示了生物货物多样性中的一种常见统计模式。我们的实验结果可以通过一个理论模型得到很好的描述，该模型将驱动蛋白 - 货物复合物与其周围环境之间的布朗相关动摩擦与观察到的货物速度的通用伽马分布联系起来。该模型阐明了控制细胞内货物运输的粘滑动力学，这与各种细胞过程相关，如囊泡出芽、细胞器运输和细胞迁移。
