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在微观世界中研究高氢气饱和度下微生物反应的实验程序。

Experimental procedures for studying microbial reactions under high hydrogen gas saturations in microcosms.

作者信息

Jaques Aidan, Ireland Mark, van der Land Cees, Dirmeier Reinhard, Williamson Nicole, Christgen Beate

机构信息

School of Natural and Environmental Sciences, Newcastle University, England, United Kingdom.

BP Biosciences Center, San Diego, USA.

出版信息

MethodsX. 2025 May 10;14:103344. doi: 10.1016/j.mex.2025.103344. eCollection 2025 Jun.

DOI:10.1016/j.mex.2025.103344
PMID:40475891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12139486/
Abstract

This methodology is proposed to investigate the response of microbial communities through analysis of headspace composition under high saturations of hydrogen. Changes in headspace composition will be related to specific communities and environmental conditions that will influence their response and result in changes in gases produced or potential changes in the liquid phase of microcosms pertaining to the hydrogen consumption rate through microbial metabolic processes. A step-by-step procedure is documented here.•Methodology includes an easy setup utilising common laboratory equipment.•The method showed minor appreciable loss of hydrogen from the microcosm setup/storage and the use of exetainers for gas measurements.•Actively studied microbial hydrogen consumption across 18 days at 30 °C and 50 °C This method is useful for the first instances in scientific studies towards understanding species or microbial communities found in environments with high percentages of hydrogen: underground hydrogen storage sites, hydrogen pipelines, and hydrogen leakage into subsurface soils.

摘要

本方法旨在通过分析高氢饱和度下顶空成分来研究微生物群落的响应。顶空成分的变化将与特定群落和环境条件相关，这些条件会影响它们的响应，并导致产生的气体变化或与微生物代谢过程中氢消耗率相关的微观世界液相的潜在变化。此处记录了一个逐步的程序。

  • 方法包括使用常见实验室设备的简易设置。

  • 该方法显示微观世界设置/储存中氢气的损失较小且可察觉，并且使用顶空采样器进行气体测量。

  • 在30°C和50°C下对微生物的氢消耗进行了为期18天的积极研究。

该方法对于科学研究中首次了解存在高比例氢气的环境（如地下储氢场所、氢气管道以及氢气泄漏到地下土壤中）中发现的物种或微生物群落很有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/b0a7053ed99e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/57b8d677a8e4/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/4e85e4525c55/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/2ed34e3bd988/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/41a421d79ffa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/b0a7053ed99e/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/57b8d677a8e4/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/4e85e4525c55/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/2ed34e3bd988/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/41a421d79ffa/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/59b1/12139486/b0a7053ed99e/gr4.jpg

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