Dhillon Arshminder Kaur, Jhilta Agrim, Ladhi Ritu, Bagdwal Harshita, Singh Ashi, Verma Rahul K, Singh Monika
Institute of Nano Science and Technology, Knowledge City, Sector-81, Mohali-140306, Punjab, India.
Department of Chemistry, Indian Institute of Technology, Delhi, India.
Nanoscale. 2025 Jun 19;17(24):14630-14636. doi: 10.1039/d5nr00785b.
Integration of novel biocompatible nanostructures as effective sensing platforms is still of great significance for robust and rapid analysis. Herein, novel iron(III) metal-organic framework (Fe-MOF) nanospheres with the formula [Fe(μ-O)(COH)(COO)(HO)·2DMF] were successfully developed and characterized by single crystal X-ray diffraction (SXRD) studies. Interestingly, fluorescence studies reveal that Fe-MOF is an effective multi-functional turn-on sensor for nicotine and cotinine that exhibits a blue shift, a low detection limit, notable recyclability, and good anti-interference ability. This material satisfies essential biological criteria by being minimally toxic to living cells (L929 and A549 cell lines) and retaining structural stability in water across a broad pH range (3-11), enabling its successful application in biological imaging of nicotine and cotinine in living cells. To our knowledge, this is the first report of fluorescence enhancement for cotinine detection with a metal-organic framework. Density functional theory (DFT) calculations and ultraviolet-visible (UV-vis) absorption studies have elucidated the plausible sensing mechanism. This study significantly expands the potential of Fe-MOF in sensing and biological applications.
将新型生物相容性纳米结构整合为有效的传感平台对于强大而快速的分析仍然具有重要意义。在此，成功开发了具有化学式[Fe(μ-O)(COH)(COO)(HO)·2DMF]的新型铁(III)金属有机框架(Fe-MOF)纳米球，并通过单晶X射线衍射(SXRD)研究对其进行了表征。有趣的是，荧光研究表明，Fe-MOF是一种用于尼古丁和可替宁的有效的多功能开启型传感器，具有蓝移、低检测限、显著的可回收性和良好的抗干扰能力。这种材料对活细胞（L929和A549细胞系）毒性极小，并在较宽的pH范围（3-11）内于水中保持结构稳定性，满足了基本的生物学标准，从而能够成功应用于活细胞中尼古丁和可替宁的生物成像。据我们所知，这是首次报道用金属有机框架检测可替宁时荧光增强的情况。密度泛函理论(DFT)计算和紫外可见(UV-vis)吸收研究阐明了可能的传感机制。这项研究显著扩展了Fe-MOF在传感和生物应用方面的潜力。
